• 建立在新設計上的高靈敏度
• 更快的成像速度支持新的生物學應用
• 隨時根據需要成長的共聚焦系統

Leica TCS SP8 – 創新點概覽

STED Key Publications

First publication on STED technology:

Hell, S. W. & Wichmann, J. Breaking the diffraction resolution limit by stimulated emission: stimulated-emission-depletion fluorescence microscopy. Optics letters 19, 780-782, (1994).http://www.ncbi.nlm.nih.gov/pubmed/19844443

First biological image:

Klar, T. A., Jakobs, S., Dyba, M., Egner, A. & Hell, S. W. Fluorescence microscopy with diffraction resolution barrier broken by stimulated emission. Proceedings of the National Academy of Sciences of the United States of America 97, 8206-8210, (2000). http://www.ncbi.nlm.nih.gov/pubmed/10899992

STED with genetically encoded marker: GFP

Willig, K. I. et al. Nanoscale resolution in GFP-based microscopy. Nature methods 3, 721-723, (2006).http://www.ncbi.nlm.nih.gov/pubmed/16896340

STED with continuous wave laser beams:

Willig, K. I., Harke, B., Medda, R. & Hell, S. W. STED microscopy with continuous wave beams. Nature methods 4, 915-918, (2007). http://www.ncbi.nlm.nih.gov/pubmed/17952088

Two-color STED with two STED lines

Donnert, G. et al. Two-color far-field fluorescence nanoscopy. Biophysical journal 92, L67-69, (2007).http://www.ncbi.nlm.nih.gov/pubmed/17307826

Iso-STED and two-color STED with one STED line

Schmidt, R. et al. Spherical nanosized focal spot unravels the interior of cells. Nature methods 5, 539-544, (2008).http://www.ncbi.nlm.nih.gov/pubmed/18488034

STED-FCS application

Eggeling, C. et al. Direct observation of the nanoscale dynamics of membrane lipids in a living cell. Nature 457, 1159-1162, (2009). http://www.ncbi.nlm.nih.gov/pubmed/19098897

Gated STED

Vicidomini, G. et al. Sharper low-power STED nanoscopy by time gating. Nature methods 8, 571-573, (2011).http://www.ncbi.nlm.nih.gov/pubmed/21642963

Selected Publications with Leica STED instrument

2016

Qiu, Z. et al. Translation Microscopy (TRAM) for super-resolution imaging. Scientific reports 6, 19993, (2016).http://www.ncbi.nlm.nih.gov/pubmed/26822455

Ruge, C. A. et al. Disintegration of nano-embedded microparticles after deposition on mucus: A mechanistic study. Colloids and surfaces. B, Biointerfaces 139, 219-227, (2016). http://www.ncbi.nlm.nih.gov/pubmed/26720142

Scheuring, D. et al. Actin-dependent vacuolar occupancy of the cell determines auxin-induced growth repression. Proceedings of the National Academy of Sciences of the United States of America 113, 452-457, (2016).http://www.ncbi.nlm.nih.gov/pubmed/26715743

Torreno-Pina, J. A. et al. The actin cytoskeleton modulates the activation of iNKT cells by segregating CD1d nanoclusters on antigen-presenting cells. Proceedings of the National Academy of Sciences of the United States of America 113, E772-781, (2016). http://www.ncbi.nlm.nih.gov/pubmed/26798067