O2微電極

Electrode Assembly and Preparation
1. Remove the acrylic housing from the Oxygen electrode by unscrewing it from the electrode body.
This housing, with affixed Teflon membrane, does not contain the necessary oxygen electrolyte
solution.
2. Add oxygen electrolyte solution to the acrylic housing. This is accomplished by attaching a filling
tip to the bottle of electrolyte and adding the electrolyte to the housing to a minimum height of 6
mm. Gently place the bubble-free end of the filling tip against the Teflon membrane and release the
electrolyte to the minimum height.
3. Insert the housing on to the Oxygen electrode, being careful not to trap any air bubbles near the
electrode tip. The housing is screwed clockwise into the body of the electrode until it stops. Check
the tip of the electrode for proper seating of the housing against the electrode. The electrode is
properly seated if the electrode protrudes slightly beyond the end of the housing.

O2微電極

Calibration
Calibration of the electrode requires the use of two standard gases with percent values that are close to
the percent values of Oxygen to be measured. Common values used are 0% Oxygen for zeroing and 21%
Oxygen (Ambient Air) for sloping or gain

Handling
When necessary, the membrane of the electrode can be replaced by following the assembly procedure
above. When removing and replacing a membrane as well as when calibrating or making measurements,
be careful not to apply pressure against the internal electrode. Any excessive pressure against the
internal electrode can cause the electrode to crack rendering it useless and unrepeatable
Cleaning
When using the electrode in solutions containing protein, the electrode should be soaked in an enzyme
cleaning solution such as Terg-a-zyme (Alconox, Inc.) after each use for a couple of minutes to remove
the protein from the membrane surface. This will prolong the useful life of the membrane.