Recombinant Beclin 1 (BECN1) 

芐氯素1(BECN1)重組蛋白

[ PROPERTIES ]

Residues: Met1~Thr273,with two N-terminal Tags, His-tag and T7-tag.

Host: E. coli

Subcellular Location: Golgi apparatus,trans-Golgi network membrane; Peripheralmembrane protein.

Purity: >95%

Endotoxin Level: <1.0EU per 1μg

(determined by the LAL method).

Formulation: Supplied as lyophilized form in PBS,pH7.4, containing 5% trehalose, 0.01% sarcosyl.

Predicted isoelectric point: 4.7

Predicted Molecular Mass: 35.2kDa

Applications: SDS-PAGE; WB; ELISA; IP.

(May be suitable for use in other assays to be determined by the end user.)

[ USAGE ]

Reconstitute in sterile PBS, pH7.2-pH7.4.

[ STORAGE AND STABILITY ]

Storage: Avoid repeated freeze/thaw cycles.

           Store at 2-8oC for one month.

           Aliquot and store at -80oC for 12 months.

Stability Test: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

[SEQUENCE ]

The target protein is fused with two N-terminal Tags, His-tag and T7-tag, itssequence is listed below.

MGSSHHHHHH SSGLVPRGSH MASMTGGQQM GRGSEF-MEGSKTSNNS TMQVSFVCQRCSQPLKLDTS FKILDRVTIQ ELTAPLLTTA QAKPGETQEE ETNSGEEPFI ETPRQDGVSRRFIPPARMMS TESANSFTLI GEASDGGTME NLSRRLKVTG DLFDIMSGQT DVDHPLCEECTDTLLDQLDT QLNVTENECQ NYKRCLEILE QMNEDDSEQL QMELKELALE EERLIQELEDVEKNRKIVAE NLEKVQAEAE RLDQEEAQYQ REYSEFKRQ LELDDELKSV ENQMRYAQTQLDKLKKTNVF NAT

[  IDENTIFICATION  ]

特別提示:本公司的所有產品僅可用于科研實驗,嚴禁用于臨床醫療及其他非科研用途!以實際收貨產品說明書為準,網站說明書僅供參考！