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Annexin V/PI法是目前較為流行的檢測細胞凋亡的方法。AnnexinV是一種鈣依賴性的磷脂結(jié)合蛋白，對磷脂酰絲氨酸（phosphatidyl-serine,PS）具有較高的親和性，它可以較為敏感的檢測細胞膜表面的翻轉(zhuǎn)的PS。將Annexin-V進行熒光素（FITC、PE）標記，以標記的Annexin-V作為熒光探針，利用流式細胞或熒光顯微鏡可檢測細胞凋亡的發(fā)生。該方法快速可靠，不需要固定細胞，從而避免了Tunel法因固定而出現(xiàn)的DNA 段丟失，該方法廣泛用于細胞的早期凋亡檢測。

         Balb/c 小鼠脾細胞流式染色（Southern biotech  cat#10010-02）

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應(yīng)用文獻：

• Corey Speers,etal. Maternal Embryonic Leucine Zipper Kinase (MELK) as a Novel Mediator and Biomarker of Radioresistance in Human Breast Cancer. Clinical Cancer Research..（應(yīng)用產(chǎn)品：cat#10010-02）.
• Mallikarjun Handigund,etal. Evaluation of in vitro storage characteristics of cold stored platelet concentrates with N acetylcysteine (NAC). Transfusion and Apheresis Science. Published Feb（應(yīng)用產(chǎn)品： cat#10010-02.）
• G Feng,etal..Interleukin21 mediates hepatitis B virus‐associated liver cirrhosis by activating hepatic stellate cells.（應(yīng)用產(chǎn)品： cat#10010-09）.
• Ruonan Xu,etal. Complement 5a stimulates hepatic stellate cells in vitro, and is increased in the plasma of patients with chronic hepatitis B. Immunology. （應(yīng)用產(chǎn)品： cat#10010-09）
• Zhu, Jin-Hui,etal.Suppression of Cellular Apoptosis Susceptibility (CSE1L) Inhibits Proliferation and Induces Apoptosis in Colorectal Cancer Cells. Cancer Prevetion. （應(yīng)用產(chǎn)品： cat#10010-09）