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SpeciesReactivity
Mouse

Form
Stds=50-1000 ng/ml; Sample=100 ul (diluted); Sensitivity= 25 ng/ml; 105 min assay; Quantitative, Sandwich, 96 tests

Samples
Serum. Plasma, Culture medium and other fluids may be adapted

KitContents
1. Anti-Mouse IgG3 coated strip plate (8 wells x 12 strips) 6361 2. Mouse IgG3 Std. A (0 ng/mL), 0.22 mL 6362 3. Mouse IgG3 Std. B (100 ng/mL), 0.22 mL 6363 4. Mouse IgG3 Std. C (250 ng/mL), 0. 22 mL 6364 5. Mouse IgG3 Std. D (500 ng/mL), 0. 22 mL 6365 6. Mouse IgG3 Std. E (1000 ng/mL), 0. 22 mL 6366 7. Mouse IgG3 Std. F (2000 ng/mL), 0. 22 mL 6367 8. Wash Buffer (100X), 10 mL WB-100 9. Sample Diluent (20x), 10 mL SD-20 10. Anti-Mouse IgG3 HRP Conjugate (100x), 0.12 mL 6368 11. TMB Substrate, 12 mL 80091 12. Stop solution, 12 mL 80101 13. Instruction Manual M-6360

SpCrossreactivity
The antibodies used in this kit are specific for IgG3 with no significant reactivity to IgG1-2, or IgA, IgM, or IgE. Mouse IgG3 ELISA kit species crossreactivity was tested with the following animal serum at dilutions of 1:100: Significant cross-reactivity was observed with rat (200%), and hamster (22%) serum. Guinea Pig, Rabbit, Human, Monkey, Goat, and Chicken serum had less than 1% cross-reactivity.

MSDS
TMB (substrate), H2SO4 (1% in a buffered stop solution), and Prolcin-300 (0.1% v/v in standards, sample diluent and HRP-conjugates

For research use only, not for diagnostic or therapeutic use.