北京西美杰科技有限公司
主營產品: 生物學檢測試劑盒,化學試劑,蛋白質氧化損傷,抗原抗體 |
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18210960361
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- 西美杰
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- 400-050-4006
- 手機:
- 18210960361
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- 010-88597838
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[供應]A3418,0050-DNA - Isolation reagent for genomic DNA(基因組DNA提取試)
- 貨物所在地:
- 北京北京市
- 產地:
- 德國
- 更新時間:
- 2024-09-03 21:00:03
- 有效期:
- 2024年9月3日--2025年3月3日
- 已獲點擊:
- 259
【簡單介紹】DNA - Isolation reagent for genomic DNA,A3418,0050,50 ml
【詳細說明】
HS-No. | 38220000 | |||
---|---|---|---|---|
Storage | RT | |||
• Phenol-free solution for the rapid isolation of genomic DNA | ||||
• from samples of human, animal (incl. mouse tail), plant, yeast, bacterial and viral origin | ||||
R | 20/21/22-32 | |||
S | 24/25 | |||
harmful |
(1) Chomczynski, P. & Sacchi, N. (1987) Anal. Biochem. 162, 156-159
Single-step method of RNA isolation by "AGPC" extraction.
The Genomic DNA Isolation Reagent is a non-organic and ready-to-use reagent for the isolation of genomic DNA from human, animal (incl. mouse tail), plant, yeast, bacterial and viral origin. It is an improved version of the Chomczynski method (1), which is based on disruption of cells in a guanidinium-detergent lysing solution that hydrolyzes RNA and allows the selective precipitation of DNA from a cell lysate with ethanol. Following an ethanol wash, DNA is solubilzed in water or 8 mM NaOH. There is no phenol in the Genomic DNA Isolation Reagent. The protocol is fast and permits isolation of genomic DNA from a large number of samples of small or large volumes. The procedure can be completed in 10 - 30 minutes with DNA recovery of 70 - 100 %. The isolated DNA can be used, without additional purification, for Southern-analysis, dot blot hybridization, molecular cloning, RFLP, PCR and other molecular biology and biotechnology applications. A detailed application protocol is included.