詳細介紹
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產(chǎn)品名稱:人胰島素樣生長因子結(jié)合蛋白4
英文名稱:Human insulin-like growth factors binding protein 4,IGFBP-4 ELISA KIT
產(chǎn)品規(guī)格:96人份/48人份
主要組成成分:試劑盒
性狀:液體
適應(yīng)癥:科研
貯藏:2-8℃
Steps description(英文)
Standard, Sample diluent |
Add Standard, Sample diluent, incubate for 30 min at |
Wash 5 time,Add HRP-Conjugate reagent, incubate for 30 min at |
Wash 5 times,Add Chromogen Solution A and B, incubate for 30 min at |
Add Stopp Solution |
Read absorbance at 450nm within 15 min |
calculate |
Calculate
Take the standard density as the horizontal, the OD value for the vertical ,draw the standard curve on graph paper, Find out the corresponding density according to the sample OD value by the Sample curve, multiplied by the dilution multiple, or calculate the straight line regression equation of the standard curve with the standard density and the OD value ,with the sample OD value in the equation, calculate the sample density, multiplied by the dilution factor, the result is the sample actual density.
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