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上海起發(fā)為以色列alomone授權(quán)一級(jí)代理,*。
Alomone labs公司是一家位于耶路撒冷的以色列的的生物科技公司,專著于提供神經(jīng)生物學(xué)和細(xì)胞信號(hào)傳導(dǎo)產(chǎn)品,如離子通道、G蛋白、神經(jīng)調(diào)節(jié)因子等,是離子通道研究的專家。
Alomone ASR-036-F說(shuō)明書(shū)
5-Hydroxytryptamine (Serotonin) receptor 4, Serotonin receptor 4, HTR4
5-羥色胺(5-羥色胺)受體4,5-羥色胺受體4,HTR4
貨號(hào):ASR-036-F
來(lái)源:兔子
類型:多克隆
應(yīng)用:FC
也可以在:ICC,LCI
反應(yīng)性:H,M,R
CBE-基于細(xì)胞的ELISA,F(xiàn)C-流式細(xì)胞術(shù),ICC-免??疫細(xì)胞化學(xué),IE-間接ELISA,IFC-間接流式細(xì)胞術(shù),IHC-免疫組織化學(xué),IP-免疫沉淀,LCI-活細(xì)胞成像,N-中和,WB-蛋白質(zhì)印跡
H-人,M-小鼠,R-大鼠
抗5HT4受體(細(xì)胞外)抗體 (#ASR-036)是針對(duì)大鼠蛋白表位的高度特異性抗體。該抗體可用于免疫印跡,免疫細(xì)胞化學(xué)和活細(xì)胞成像應(yīng)用。它被設(shè)計(jì)用于識(shí)別來(lái)自大鼠,小鼠和人類樣品的5-羥色胺受體4。
抗5HT4受體(細(xì)胞外)-FITC抗體 (#ASR-036-F)與異硫氰酸熒光素(FITC)直接綴合。該抗體可用于免疫熒光應(yīng)用,例如直接活細(xì)胞流式細(xì)胞術(shù)。
直接流式細(xì)胞儀
活完整小鼠J774巨噬細(xì)胞的直接流式細(xì)胞術(shù):
___ 細(xì)胞。
___ 細(xì)胞+兔IgG同種型對(duì)照-FITC。
___ 細(xì)胞+抗5HT4受體(細(xì)胞外)-FITC抗體 (#ASR-036-F),(2.5μg)。
活體完整人THP-1單核細(xì)胞白血病細(xì)胞的直接流式細(xì)胞術(shù):
___ 細(xì)胞。
___ 細(xì)胞+兔IgG同種型對(duì)照-FITC。
___ 細(xì)胞+抗5HT4受體(細(xì)胞外)-FITC抗體 (#ASR-036-F),(5μg)。
免疫原
肽(C)DVIEKRKFNHNSN,對(duì)應(yīng)于大鼠5-羥色胺受體4(登錄號(hào)Q62758)的氨基酸殘基168-180 。2 第二 胞外環(huán)。
同源小鼠 - 12/13氨基酸殘基相同; 人類 - 11/13氨基酸殘基相同。
純度親和力在固定的抗原上純化。
配方凍干粉。重構(gòu)抗體含有磷酸鹽緩沖鹽水(PBS),pH 7.4,1%BSA,0.05%NaN 3。
標(biāo)簽熒光素異硫氰酸酯(FITC)。
每批的標(biāo)準(zhǔn)質(zhì)量控制Western印跡分析(未標(biāo)記的抗體,#ASR-036)和直接流式細(xì)胞術(shù)(標(biāo)記的抗體)。
肽確認(rèn)通過(guò)氨基酸分析和質(zhì)譜確認(rèn)。
重建前儲(chǔ)存抗體在室溫下作為凍干粉末運(yùn)輸。抵達(dá)后,應(yīng)儲(chǔ)存在-20°C。
重構(gòu)50μl雙蒸水(DDW)。
重構(gòu)后的抗體濃度為1mg / ml。
重建后儲(chǔ)存重組溶液可在4°C下保存,避光,長(zhǎng)可保存1周。較長(zhǎng)時(shí)間,小等分試樣應(yīng)儲(chǔ)存在-20°C。避免多次凍融。使用前離心所有抗體制劑(10000×g 5分鐘)。
重建前控制抗原儲(chǔ)存凍干粉末可在室溫下完整保存兩周。較長(zhǎng)時(shí)間,應(yīng)儲(chǔ)存在-20°C。
對(duì)照抗原重建100μl雙蒸水(DDW)。
在重構(gòu)-20℃后控制抗原儲(chǔ)存。
預(yù)吸附控制每1μg抗體1μg肽。
科學(xué)背景
5-羥色胺受體4(5-HT4受體)是介導(dǎo)中樞和外周靶標(biāo)中5-羥色胺信號(hào)傳導(dǎo)的大家族受體中的一個(gè)亞類。5-HT4受體激活在學(xué)習(xí)和記憶,攝食控制和應(yīng)激反應(yīng)中起作用。5-HT4受體由復(fù)合基因編碼,該復(fù)合基因在人類中產(chǎn)生十種不同的剪接變體1,2。
5-HT4受體是跨越7個(gè)跨膜G蛋白偶聯(lián)受體(GPCR)的成員,并通過(guò)G s偶聯(lián) 以增加cAMP產(chǎn)生。GPCR具有相似的拓?fù)浣Y(jié)構(gòu),七個(gè)跨膜螺旋(TMH)通過(guò)三個(gè)細(xì)胞外環(huán)(ECL)連接,三個(gè)細(xì)胞內(nèi)環(huán)(ICL); N末端位于膜的細(xì)胞外側(cè),C末端是細(xì)胞質(zhì)3。
在中樞神經(jīng)系統(tǒng)內(nèi),這些受體在邊緣結(jié)構(gòu)中表達(dá),包括:下丘腦,伏核,前額葉皮質(zhì),杏仁核和海馬,它們有助于情緒和認(rèn)知功能4。
低水平的5-HT4受體與許多神經(jīng)精神疾病有關(guān),包括阿爾茨海默病,亨廷頓舞蹈病,喂養(yǎng)障礙和壓力相關(guān)疾病,如焦慮和抑郁5。
貨號(hào) | 品名 | 規(guī)格 | 目錄價(jià) | 品牌 |
A-105 | A-803467 | 10 mg | 592 | Alomone |
A-105 | A-803467 | 25 mg | 1520 | Alomone |
A-105 | A-803467 | 50 mg | 2752 | Alomone |
A-105 | A-803467 | 100 mg | 5072 | Alomone |
A-110 | Amlodipine | 100 mg | 656 | Alomone |
A-110 | Amlodipine | 250 mg | 1184 | Alomone |
A-110 | Amlodipine | 1 g | 2416 | Alomone |
A-115 | 4-Aminopyridine | 25 g | 480 | Alomone |
A-115 | 4-Aminopyridine | 100 g | 1184 | Alomone |
A-120 | Amlodipine besylate | 10 mg | 384 | Alomone |
A-120 | Amlodipine besylate | 25 mg | 480 | Alomone |
A-120 | Amlodipine besylate | 50 mg | 656 | Alomone |
A-120 | Amlodipine besylate | 100 mg | 1008 | Alomone |
A-120 | Amlodipine besylate | 500 mg | 1392 | Alomone |
A-120 | Amlodipine besylate | 1 g | 1712 | Alomone |
A-120 | Amlodipine besylate | 5 g | 5232 | Alomone |
A-135 | Azelnidipine | 5 mg | 656 | Alomone |
A-135 | Azelnidipine | 10 mg | 832 | Alomone |
A-135 | Azelnidipine | 25 mg | 1536 | Alomone |
A-135 | Azelnidipine | 50 mg | 2768 | Alomone |
A-140 | Amiloride hydrochloride | 1 g | 480 | Alomone |
A-140 | Amiloride hydrochloride | 5 g | 2064 | Alomone |
A-140 | Amiloride hydrochloride | 10 g | 3648 | Alomone |
A-145 | Ambroxol hydrochloride | 1 g | 304 | Alomone |
A-145 | Ambroxol hydrochloride | 5 g | 480 | Alomone |
A-150 | Aconitine | 25 mg | 432 | Alomone |
A-150 | Aconitine | 50 mg | 784 | Alomone |
A-150 | Aconitine | 100 mg | 1392 | Alomone |
A-150 | Aconitine | 250 mg | 2768 | Alomone |
A-155 | Amitriptyline hydrochloride | 10 g | 480 | Alomone |
A-155 | Amitriptyline hydrochloride | 25 g | 832 | Alomone |
A-155 | Amitriptyline hydrochloride | 100 g | 2944 | Alomone |
A-165 | Amentoflavone | 1 mg | 752 | Alomone |
A-165 | Amentoflavone | 5 mg | 1552 | Alomone |
A-165 | Amentoflavone | 10 mg | 2192 | Alomone |
A-165 | Amentoflavone | 25 mg | 4368 | Alomone |
A-180 | AMG 9810 | 5 mg | 656 | Alomone |
A-180 | AMG 9810 | 10 mg | 832 | Alomone |
A-180 | AMG 9810 | 25 mg | 1888 | Alomone |
A-180 | AMG 9810 | 50 mg | 3488 | Alomone |
A-190 | AM 404 | 5 mg | 480 | Alomone |
A-190 | AM 404 | 10 mg | 656 | Alomone |
A-190 | AM 404 | 25 mg | 1536 | Alomone |
A-190 | AM 404 | 50 mg | 2592 | Alomone |
A-195 | A-889425 | 1 mg | 1072 | Alomone |
A-195 | A-889425 | 5 mg | 2416 | Alomone |
A-195 | A-889425 | 10 mg | 3296 | Alomone |
A-195 | A-889425 | 25 mg | 7520 | Alomone |
A-195 | A-889425 | 50 mg | 13824 | Alomone |
A-205 | 3-AQC | 5 mg | 880 | Alomone |
A-205 | 3-AQC | 10 mg | 1392 | Alomone |
A-205 | 3-AQC | 25 mg | 3152 | Alomone |
A-205 | 3-AQC | 50 mg | 5712 | Alomone |
A-215 | ANA-12 | 5 mg | 752 | Alomone |
A-215 | ANA-12 | 10 mg | 1072 | Alomone |
A-215 | ANA-12 | 25 mg | 2448 | Alomone |
A-215 | ANA-12 | 50 mg | 4400 | Alomone |
A-215 | ANA-12 | 100 mg | 7920 | Alomone |
A-225 | A 967079 | 5 mg | 592 | Alomone |
A-225 | A 967079 | 10 mg | 1072 | Alomone |
A-225 | A 967079 | 25 mg | 2448 | Alomone |
A-225 | A 967079 | 50 mg | 4592 | Alomone |
A-225 | A 967079 | 100 mg | 8592 | Alomone |
A-235 | Alosetron | 5 mg | 432 | Alomone |
A-235 | Alosetron | 10 mg | 592 | Alomone |
A-235 | Alosetron | 25 mg | 1072 | Alomone |
A-235 | Alosetron | 50 mg | 1872 | Alomone |
A-236 | Alosetron hydrochloride | 10 mg | 432 | Alomone |
A-236 | Alosetron hydrochloride | 25 mg | 992 | Alomone |
A-236 | Alosetron hydrochloride | 50 mg | 1808 | Alomone |
A-240 | Azasetron | 5 mg | 1392 | Alomone |
A-240 | Azasetron | 10 mg | 2352 | Alomone |
A-240 | Azasetron | 25 mg | 5312 | Alomone |
A-240 | Azasetron | 50 mg | 9552 | Alomone |
A-245 | Azilsartan | 10 mg | 592 | Alomone |
A-245 | Azilsartan | 25 mg | 1328 | Alomone |
A-245 | Azilsartan | 100 mg | 4272 | Alomone |
A-245 | Azilsartan | 1 g | 5552 | Alomone |
A-250 | AP-18 | 5 mg | 592 | Alomone |
A-250 | AP-18 | 10 mg | 752 | Alomone |
A-250 | AP-18 | 25 mg | 1712 | Alomone |
A-250 | AP-18 | 50 mg | 3152 | Alomone |
A-260 | alpha-Asarone | 1 g | 432 | Alomone |
A-260 | alpha-Asarone | 5 g | 2032 | Alomone |
A-260 | alpha-Asarone | 10 g | 2672 | Alomone |
A-265 | (RS)-AMPA | 5 mg | 1072 | Alomone |
A-265 | (RS)-AMPA | 10 mg | 1392 | Alomone |
A-265 | (RS)-AMPA | 25 mg | 3152 | Alomone |
A-265 | (RS)-AMPA | 50 mg | 5712 | Alomone |
A-266 | (RS)-AMPA hydrobromide | 1 mg | 592 | Alomone |
A-266 | (RS)-AMPA hydrobromide | 5 mg | 1392 | Alomone |
A-266 | (RS)-AMPA hydrobromide | 10 mg | 2032 | Alomone |
A-266 | (RS)-AMPA hydrobromide | 25 mg | 4560 | Alomone |
A-266 | (RS)-AMPA hydrobromide | 50 mg | 8208 | Alomone |
A-267 | (S)-AMPA | 1 mg | 592 | Alomone |
A-267 | (S)-AMPA | 5 mg | 1072 | Alomone |
A-267 | (S)-AMPA | 10 mg | 1872 | Alomone |
A-267 | (S)-AMPA | 25 mg | 4208 | Alomone |
A-267 | (S)-AMPA | 50 mg | 7568 | Alomone |
A-280 | trans-ACBD | 5 mg | 1392 | Alomone |
A-280 | trans-ACBD | 10 mg | 2192 | Alomone |
A-280 | trans-ACBD | 25 mg | 4912 | Alomone |
A-281 | cis-ACBD | 5 mg | 752 | Alomone |
A-281 | cis-ACBD | 10 mg | 1072 | Alomone |
A-281 | cis-ACBD | 25 mg | 2448 | Alomone |
A-281 | cis-ACBD | 50 mg | 4432 | Alomone |
A-285 | Arcaine sulfate | 25 mg | 592 | Alomone |
A-285 | Arcaine sulfate | 50 mg | 752 | Alomone |
A-285 | Arcaine sulfate | 100 mg | 1392 | Alomone |
A-300 | AMG-9090 | 5 mg | 1232 | Alomone |
A-300 | AMG-9090 | 10 mg | 1712 | Alomone |
A-300 | AMG-9090 | 25 mg | 3152 | Alomone |
A-300 | AMG-9090 | 50 mg | 4432 | Alomone |
A-305 | AMTB hydrochloride | 5 mg | 592 | Alomone |
A-305 | AMTB hydrochloride | 10 mg | 1072 | Alomone |
A-305 | AMTB hydrochloride | 25 mg | 2352 | Alomone |
A-305 | AMTB hydrochloride | 50 mg | 3792 | Alomone |
A-330 | (S)-ATPO | 5 mg | 3792 | Alomone |
A-330 | (S)-ATPO | 10 mg | 6832 | Alomone |
A-330 | (S)-ATPO | 25 mg | 15312 | Alomone |
A-335 | Arvanil | 10 mg | 432 | Alomone |
A-335 | Arvanil | 25 mg | 1008 | Alomone |
A-335 | Arvanil | 50 mg | 1872 | Alomone |
A-335 | Arvanil | 100 mg | 3312 | Alomone |
A-340 | AMG2850 | 5 mg | 2192 | Alomone |
A-340 | AMG2850 | 10 mg | 3472 | Alomone |
A-340 | AMG2850 | 25 mg | 6992 | Alomone |
A-340 | AMG2850 | 50 mg | 10352 | Alomone |
A-345 | A 784168 | 5 mg | 1072 | Alomone |
A-345 | A 784168 | 10 mg | 1872 | Alomone |
A-345 | A 784168 | 25 mg | 4208 | Alomone |
A-345 | A 784168 | 50 mg | 5648 | Alomone |
A-350 | ACT-132577 | 5 mg | 2192 | Alomone |
A-350 | ACT-132577 | 10 mg | 3792 | Alomone |
A-350 | ACT-132577 | 25 mg | 6832 | Alomone |
A-350 | ACT-132577 | 50 mg | 10768 | Alomone |
A-355 | Avosentan | 5 mg | 752 | Alomone |
A-355 | Avosentan | 10 mg | 1072 | Alomone |
A-355 | Avosentan | 25 mg | 2448 | Alomone |
A-355 | Avosentan | 50 mg | 4432 | Alomone |
A-360 | A-192621 | 1 mg | 1552 | Alomone |
A-360 | A-192621 | 5 mg | 5552 | Alomone |
A-360 | A-192621 | 10 mg | 8592 | Alomone |
A-360 | A-192621 | 25 mg | 15152 | Alomone |
A-370 | AA 29504 | 5 mg | 592 | Alomone |
A-370 | AA 29504 | 10 mg | 752 | Alomone |
A-370 | AA 29504 | 25 mg | 1712 | Alomone |
A-370 | AA 29504 | 50 mg | 3088 | Alomone |
A-375 | AF-353 | 5 mg | 1072 | Alomone |
A-375 | AF-353 | 10 mg | 1872 | Alomone |
A-375 | AF-353 | 25 mg | 4208 | Alomone |
A-375 | AF-353 | 50 mg | 7568 | Alomone |
A-380 | A-317491 sodium salt hydrate | 5 mg | 1072 | Alomone |
A-380 | A-317491 sodium salt hydrate | 10 mg | 1712 | Alomone |
A-380 | A-317491 sodium salt hydrate | 25 mg | 3888 | Alomone |
A-380 | A-317491 sodium salt hydrate | 50 mg | 6032 | Alomone |
A-390 | A-850002 | 5 mg | 1552 | Alomone |
A-390 | A-850002 | 10 mg | 2192 | Alomone |
A-390 | A-850002 | 25 mg | 4368 | Alomone |
A-390 | A-850002 | 50 mg | 7888 | Alomone |
A-400 | Anthopleurin-C | 5 µg | 1728 | Alomone |
A-400 | Anthopleurin-C | 5 x 5 µg | 6896 | Alomone |
A-400 | Anthopleurin-C | 10 µg | 2240 | Alomone |
A-400 | Anthopleurin-C | 5 x 10 µg | 8944 | Alomone |
A-400 | Anthopleurin-C | 0.1 mg | 8368 | Alomone |
A-435 | A-794278 | 5 mg | 2192 | Alomone |
A-435 | A-794278 | 10 mg | 3472 | Alomone |
A-435 | A-794278 | 25 mg | 6064 | Alomone |
A-435 | A-794278 | 50 mg | 8544 | Alomone |
A-440 | A-841720 | 5 mg | 1072 | Alomone |
A-440 | A-841720 | 10 mg | 1712 | Alomone |
A-440 | A-841720 | 25 mg | 3472 | Alomone |
A-440 | A-841720 | 50 mg | 5552 | Alomone |
A-455 | Atrasentan | 1 mg | 752 | Alomone |
A-455 | Atrasentan | 5 mg | 2032 | Alomone |
A-455 | Atrasentan | 10 mg | 2672 | Alomone |
A-455 | Atrasentan | 25 mg | 6000 | Alomone |
A-455 | Atrasentan | 50 mg | 9552 | Alomone |
A-510 | Anhydroryanodine | 50 µg | 3296 | Alomone |
A-520 | Anisomycin | 5 mg | 272 | Alomone |
A-520 | Anisomycin | 10 mg | 368 | Alomone |
A-520 | Anisomycin | 25 mg | 752 | Alomone |
A-520 | Anisomycin | 50 mg | 1072 | Alomone |
A-520 | Anisomycin | 100 mg | 1968 | Alomone |
A-580 | 17-AAG | 0.1 mg | 1728 | Alomone |
A-580 | 17-AAG | 5 x 0.1 mg | 6896 | Alomone |
A-600 | A23187 | 1 mg | 480 | Alomone |
A-600 | A23187 | 10 x 1 mg | 2944 | Alomone |
A-600 | A23187 | 5 mg | 832 | Alomone |
A-600 | A23187 | 5 x 5 mg | 3312 | Alomone |
A-600 | A23187 | 10 mg | 1360 | Alomone |
A-800 | Ascomycin | 0.5 mg | 1184 | Alomone |
A-800 | Ascomycin | 1 mg | 1728 | Alomone |
AK-100 | P2Y Receptor Antibody Explorer Kit | 20 vials | 25360 | Alomone |
AK-101 | Adrenoceptor Antibody Explorer Kit | 18 vials | 22832 | Alomone |
AK-102 | Dopamine Receptor Antibody Explorer Kit | 12 vials | 15216 | Alomone |
AK-103 | mGluR Antibody Explorer Kit | 18 vials | 22832 | Alomone |
AK-104 | Somatostatin Receptor Antibody Explorer Kit | 12 vials | 15216 | Alomone |
AK-105 | Adenosine Receptor Antibody Explorer Kit | 8 vials | 10144 | Alomone |
AK-200 | Kir Channel Antibody Explorer Kit | 35 vials | 45648 | Alomone |
AK-201 | P2X Receptor Antibody Explorer Kit | 28 vials | 35504 | Alomone |
AK-202 | Two-Pore Domain K+ Channel Antibody Explorer Kit | 26 vials | 32976 | Alomone |
AK-203 | nAChR Antibody Explorer Kit | 26 vials | 32976 | Alomone |
AK-204 | CNGA Antibody Explorer Kit | 8 vials | 10144 | Alomone |
AK-205 | HCN Antibody Explorer Kit | 14 vials | 17760 | Alomone |
AK-206 | Muscarinic Receptor Antibody Explorer Kit | 12 vials | 15216 | Alomone |
AK-209 | Store-Operated Ca2+ Channel Antibody Explorer Kit | 19 vials | 25360 | Alomone |
AK-210 | TRPM Channel Antibody Explorer Kit | 18 vials | 22832 | Alomone |
AK-211 | TRPV Channel Antibody Explorer Kit | 24 vials | 30432 | Alomone |
AK-212 | TRPC Channel Antibody Explorer Kit | 24 vials | 30432 | Alomone |
AK-213 | AMPA Receptor Antibody Explorer Kit | 24 vials | 30432 | Alomone |
AK-214 | NMDA Receptor Antibody Explorer Kit | 16 vials | 20288 | Alomone |
AK-215 | L-Type CaV Channel Antibody Explorer Kit | 40 vials | 50720 | Alomone |
AK-216 | Non-L-Type CaV Channel Antibody Explorer Kit | 38 vials | 48192 | Alomone |
AK-217 | BKCa Channel Antibody Explorer Kit | 20 vials | 25360 | Alomone |
AK-218 | SK (Non-BKCa) Channel Antibody Explorer Kit | 17 vials | 22832 | Alomone |
AK-219 | Shaker (KV1) Channel Antibody Explorer Kit | 32 vials | 40576 | Alomone |
AK-220 | Shaker (KV)-Related Channel Antibody Explorer Kit | 34 vials | 43120 | Alomone |
AK-221 | KCNQ Channel Antibody Explorer Kit | 20 vials | 25360 | Alomone |
AK-222 | hERG and hERG-Related Channel Antibody Explorer Kit | 18 vials | 22832 | Alomone |
AK-223 | TTX-Resistant NaV Channel Antibody Explorer Kit | 26 vials | 32976 | Alomone |
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2:貨品全,現(xiàn)經(jīng)營(yíng)過(guò)700多個(gè)品牌,基本所有生物試劑耗材都可以進(jìn)口,特別是冷偏的產(chǎn)品那就更有優(yōu)勢(shì),
3:提供加急服務(wù),一般1-2周到貨,超過(guò)時(shí)限加急費(fèi)全免
4:價(jià)格公道,絕大部分價(jià)格有優(yōu)勢(shì),當(dāng)然不能保證100%產(chǎn)品都是價(jià)格低廉,因?yàn)閮r(jià)格低廉意味著沒(méi)有服務(wù).
5:良好的信譽(yù),大部分客戶我們提供貨到付款服務(wù),客戶包括清華,北大 交大 復(fù)旦,中山等100多所大學(xué),ROCHE,阿斯利康,國(guó)藥 ,fisher等500多家公司
6:我們代理的品牌有:Antibody Research Corporation,arcticzymes,Biorelevant,AmberGen, Inc. ,clemente-associates,clodronaiposomes,Columbia Biosciences,enzyme research,Gene Bridges GmbH,Genovis,AmberGen, Inc. Biotechnology GmbH,Haematologic Technologies HTI Haemtech,hookelabs,Immudex,Innovative Research of America,inspiralis ,List Biological Laboratories, Inc.,lumafluor,Microsurfaces,multiplicom,nanotools,Pel-Freez Biologicals,pentapharm,progen,Protein Ark,QA-Bio,Inc,QA-Bio,IncQuickZyme Biosciences,Teknova,TriLink BioTechnologies, Inc.,Zyagen Laboratories 等
7:我們還是invitrogen,qiagen,Midland BioProducts Corporationam,sigma;neb,roche,merck, rnd,BD, GE,pierce,BioLegend等知名*批發(fā),歡迎合作。
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