Wnt糖蛋白是存在于多種生物體內(nèi)的一種細胞外配體，Wnt作為形態(tài)發(fā)生素通過激發(fā)細胞內(nèi)遠離信號發(fā)送區(qū)域的濃度依賴反應控制胚胎形態(tài)發(fā)育。Wnt通路調(diào)節(jié)動物發(fā)育過程中的多個重要環(huán)節(jié)，例如細胞增殖、細胞遷徙及細胞分化。Wnt蛋白通過自分泌或旁分泌作用與位于細胞膜上的受體相結(jié)合，激活細胞內(nèi)信號通路調(diào)節(jié)靶基因的表達，對細胞的增殖、分化、遷移、極性化和凋亡起到重要作用。

WNT-5a,Mouse W5A-H-005,5ug 產(chǎn)品簡介
wnt通路是細胞增殖分化的關(guān)鍵調(diào)控環(huán)節(jié)，在胚胎發(fā)育和腫瘤發(fā)生中起著重要作用。wnt途徑參與了基因表達調(diào)節(jié)、細胞遷移粘附、細胞極化等過程，同時還與其它信號通路存在交叉協(xié)同。Wnt/β-catenin通路在進化過程中高度保守，此通路的主要分子構(gòu)成及相關(guān)調(diào) 控機制已得到基本闡明。對神經(jīng)系統(tǒng)而言已有足夠證據(jù)顯示此通路參與了對神經(jīng)前體細胞增殖，分化以及決定細胞命運的調(diào)控。
Wnt-3a干細胞生長分化因子是Wnt基因家族中重要成員，zui近的一些研究發(fā)現(xiàn)， Wnt-3a信號蛋白對神經(jīng)干細胞的定向分化的調(diào)控作用表現(xiàn)的十分明顯。小鼠WNT-3a 干細胞生長分化因子蛋白包含328個氨基酸。

小鼠 WNT-3a 蛋白因子，5ug 產(chǎn)品特性
? 小鼠 WNT-3a 蛋白因子，5ug 干細胞生長分化因子活性經(jīng)過NIH 3T3 細胞鑒定，純度高
? 小鼠 WNT-3a 蛋白因子，5ug 干細胞生長分化因子生物活性在NIH 3T3 細胞經(jīng)過TCF報告基因?qū)嶒炶b定The EC50為 50–150 ng/ml
? 小鼠 WNT-3a 蛋白因子，5ug 干細胞生長分化因子產(chǎn)品經(jīng)過SDS-PAGE 和HPLC雙層鑒定純度大于90%

WNT-5a,Mouse W5A-H-005,5ug 保存方法
The lyophilized protein is stable for at least 6 months if stored at -80 degreeC. Reconstituted protein is stable for at least two weeks at 4 degree C, but should be stored in aliquots at -80 degree C for longer term. Avoid repeated freeze and thaw.

WNT-5a,Mouse W5A-H-005,5ug 產(chǎn)品英文描述
WNT-3a is a member of the WNT family of signaling proteins that play a key role in embryonic development and the integrity of adult tissues. WNT-5a,Mouse W5A-H-005,5ug is a prototypic canonical WNT that signals through the β-catenin pathway. The predicted size of mouse WNT-5a,Mouse W5A-H-005,5ug is a monomeric protein containing 333 amino acid residues. Due to glycosylation, it migrates at an apparent molecular weight of 40 kDa by SDS-PAGE analysis under nonreducing conditions. StemRD’s product WNT-5a,Mouse W5A-H-005,5ug is expressed from a mouse cell line overexpressing mouse WNT-3a. Purification is performed with a proprietary process that is distinct from the published method.
?WNT-5a,Mouse W5A-H-005,5ug Purity: Greater than 90% as determined by SDS-PAGE analysis
?WNT-5a,Mouse W5A-H-005,5ug Biological Activity: The activity was determined by using a TCF reporter gene assay in NIH3T3 cells. The EC50 ranges from 50 - 150 ng/ml. Country of Origin USA
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