目錄:北京索萊寶科技有限公司>>細胞培養相關>>細胞培養試劑>> 90013DMEM 高不含酚紅谷氨酰胺丙酮酸鈉 細胞培養
供貨周期 | 現貨 | 規格 | 500ml |
---|---|---|---|
貨號 | 90013 | 應用領域 | 醫療衛生,化工,生物產業,石油 |
主要用途 | DMEM是 dulbecco's modified eagle medium的縮 |
DMEM 高不含酚紅谷氨酰胺丙酮酸鈉 細胞培養
貨號:90013
規格:500ml
別名:Dulbecco's Modified Eagle Medium
儲存條件:2-8℃
單位:瓶
DMEM 高不含酚紅谷氨酰胺丙酮酸鈉 細胞培養
DMEM是 dulbecco's modified eagle medium的縮寫,是一種含各種氨基酸和葡萄糖的培養基,是在MEM培養基的基礎上研制的。與MEM比較增加了各種成分用量,同時又分為高糖型(低于4500mg/L)和低糖型(低于1000mg/L)。高糖型有利于細胞停泊于一個位置生長,適于生長較快、附著較困難腫瘤細胞等。所以其特點主要包括以下:
(1)氨基酸含量為依格爾培養基的2倍,且含有非必需氨基酸,如甘氨酸等;
(2)維生素含量為依格爾培養基的4倍;
(3)含有糖酵解途徑中的重要物質——丙酮酸;
(4)含有微量的鐵離子.Eagle來自該培養基的發明人Harry Eagle
1640和DMEM培養基的區別
1640培養基和DMEM培養基在組分和用途上有區別,主要是組分上的區別,查各自的配方表可知。用途上這兩種培養基應用都很廣泛,且都適合很多種細胞的培養。
RPMI 1640主要用于懸浮細胞培養。可適用于哺乳動物、特殊造血細胞、正常或惡性增生的白細胞,雜交瘤細胞的培養。其它像K-562、HL-60、IM-9等成淋巴細胞、T細胞淋巴瘤細胞以及HCT-15上皮細胞等均可參考使用。
DMEM適用于貼壁細胞生長,如各種實體瘤貼壁細胞。
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