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[供應(yīng)]Y30035-Cellartis® Definitive Endoderm Differentiatio
歡迎您致電 華雅再生醫(yī)學(xué)旗艦公司：紅榮微再（上海）生物工程技術(shù)有限公司 ：152 1681 4001。華雅再生醫(yī)學(xué)-客服: 316 808 6348
Cellartis iPS Cell to Hepatocyte Differentiation System是一套Do-It-Yourself系統(tǒng)，客戶既可以對(duì)某個(gè)特殊病人來(lái)源的iPS實(shí)現(xiàn)轉(zhuǎn)化，也可以利用cellartis供應(yīng)的iPS進(jìn)行標(biāo)準(zhǔn)化操作。使用本產(chǎn)品的過(guò)程起始于誘導(dǎo)人iPS轉(zhuǎn)化成為確定性內(nèi)胚層（Definitive Endoderm (DE)）細(xì)胞，然后誘導(dǎo)轉(zhuǎn)化DE至肝細(xì)胞。本產(chǎn)品是All-In-One型產(chǎn)品，實(shí)驗(yàn)所需的培養(yǎng)基、基質(zhì)（coating）等包括在內(nèi)。應(yīng)用本產(chǎn)品轉(zhuǎn)化約3 × 106 hiPS cells,可以獲得約5 × 106 肝細(xì)胞（大約50 cm2面積的單層貼壁細(xì)胞）。
利用Cellartis iPS Cell to Hepatocyte Differentiation System轉(zhuǎn)化得到的肝細(xì)胞除了清晰表現(xiàn)出肝細(xì)胞的*特征外（如肝細(xì)胞特異性標(biāo)志物、CYP系列酶等），還保留了原始細(xì)胞供體者的遺傳基因背景。另外，相比較從組織切除獲得的原代肝臟細(xì)胞（通常需要低溫保存），通過(guò)本產(chǎn)品系統(tǒng)轉(zhuǎn)化獲得的肝細(xì)胞在更長(zhǎng)的時(shí)間跨度上保持著生理功能的穩(wěn)定性。這一點(diǎn)對(duì)于毒理學(xué)評(píng)估和病毒感染評(píng)估具有顯著的意義。為長(zhǎng)時(shí)間維持培養(yǎng)hiPS轉(zhuǎn)化而來(lái)的肝細(xì)胞，我們推薦Cellartis Hepatocyte Maintenance Medium (Cat. No. Y30051)。
關(guān)于Cellartis 的iPS Cell to Hepatocyte Differentiation技術(shù)的專項(xiàng)說(shuō)明，咨詢。
紅榮微再（上海）生物工程技術(shù)有限公司 152 1681 4001 Phone
■ Cellartis iPS Cell to Hepatocyte Differentiation System 產(chǎn)品特點(diǎn)
· Highly reproducible, robust system—the exact same protocol has been shown to work across 25 different iPS
cell lines, so no need to optimize for your lines.
· Ideal for drug metabolism and safety studies—consistently generate panels of >90% pure, functional, hiPS
cell-derived hepatocytes with diverse genetic backgrounds.
· Customized starting materials—start with any disease-relevant iPS cell lines and create accurate liver
disease models.
· Ready for personalized medicine—make patient-specific, disease-specific cells for therapeutic applications.
· Extended experimental window—hepatocytes can be used for a minimum of 11 days for functional tests.
■ 產(chǎn)品成分
· Cellartis DEF-CS 100 Culture System (Y30020, not sold separay)
· Cellartis Definitive Endoderm Differentiation Kit (Y30030, not sold separay)
· Cellartis Hepatocyte Differentiation Kit (Y30050)
歡迎您致電 華雅再生醫(yī)學(xué)旗艦公司：紅榮微再（上海）生物工程技術(shù)有限公司 ：152 1681 4001。華雅再生醫(yī)學(xué)-客服: 316 808 6348
Cellartis iPS Cell to Hepatocyte Differentiation System 產(chǎn)品訂購(gòu)清單
Y30050 Cellartis® Hepatocyte Differentiation Kit
Y30051 Cellartis® Hepatocyte Maintenance Medium
Y30035 Cellartis® Definitive Endoderm Differentiation Kit with DEF-CS™
組份-1 Cellartis DEF-CS 100 Culture System (Cat. No. Y30020
組份-2 Cellartis Definitive Endoderm Differentiation Kit (Cat. No. Y30030
"Cat. No. Y30020 Package Contents
Package 1:
· 100 ml DEF-CS 100 Basal Medium
· 800 μl DEF-CS COAT-1
Package 2:
· 300 μl DEF-CS GF-1
· 100 μl DEF-CS GF-2
· 40 μl DEF-CS GF-3
Cat. No. Y30030 Package Contents
? 10 ml Definitive Endoderm Differentiation Coating
? 18 ml Definitive Endoderm Differentiation Day 0
? 18 ml Definitive Endoderm Differentiation Day 1
? 18 ml Definitive Endoderm Differentiation Day 2
? 25 ml Definitive Endoderm Differentiation Day 3
? 47 ml Definitive Endoderm Differentiation Day 4
? 47 ml Definitive Endoderm Differentiation Day 6
歡迎您致電 華雅再生醫(yī)學(xué)旗艦公司：紅榮微再（上海）生物工程技術(shù)有限公司 ：152 1681 4001。華雅再生醫(yī)學(xué)-客服: 316 808 6348
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