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L-氨基酸氧化酶
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L-氨基酸氧化酶規(guī)格參數(shù)：
產(chǎn)品名稱（Product Name） | L-氨基酸氧化酶 |
產(chǎn)品編號(hào) （Product NO） | HZA-1103 |
英文名稱 | L-Amino Acid Oxidase from Crotalus adamanteus Venom |
CAS 編號(hào) (CAS NO) | 9000-89-9 |
存貯條件 儲(chǔ)存溫度 2-8°C產(chǎn)品介紹 L-Amino Acid Oxidase is an oligomeric glycoprotein composed of unequal amounts of two different approximay 70 kDa subunits. Three electrophoretically different isozymes occur as different combinations of the two subunits. there are approximay two moles of FAD per mole of holo-enzyme. L-amino acid oxidase catalyzes the oxidative deamination of a number of L-amino acids. The enzyme is absoluy specific for L-isomers. The Aladdin product is prepared according to Wellner and Meister, JBC, 235, 2013, (1960) to the point just prior to crystallization.別名 ;L-AAO;L-Amino acid;oxygen oxidoreductase(deaminating)生化機(jī)理 L-Amino acid oxidase is a flavoprotein with a molecular weight of 130 kDa. It consists of two different subunits of approximay 70,000 Da. Each molecule of holoenzyme has two FAD molecules. It is a glycoprotein containing about 2-5% carbohydrate, including sialic acid. Optimum pH is approximay 7.5.The enzyme may be reversibly inactivated by incubation in phosphate buffer, pH 7.5 at 38 °C. L-amino acid oxidase is involved in various metabolic pathways such as alanine and aspartate metabolism, methionine metabolism, valine, leucine and isoleucine degradation, tyrosine metabolism, phenylalanine metabolism, tryptophan metabolism, phenylalanine, tyrosine and tryptophan biosynthesis, and alkaloid biosynthesis.It occurs in many snake venoms apart from microorganisms and animal tissue, especially in kidney and liver.備注 An aqueous solution with toluene added as a preservative.應(yīng)用 L-amino acid oxidase (LAAO) is used to convert L-amino acids to their corresponding α-keto acids. L-amino acid oxidase, from Sigma, has been used in leucine aminopeptidase (LAP) activity assays.
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