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基本信息(General Information)：
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產品描述(Description)：
| Analysis Note | The enzyme can be initially dissolved in 50 mM Hepes/KOH pH 7.4, 150 mM NaCl at 10 mg/ml. Further dilutions should be made in the cell culture media most appropriate for the cells being dissociated. Dispase is a metalloproteinase and is activated by dival |
| Application | The enzyme from Beyotime has been used in developing a protocol for ex vivo culture of mouse embryonic mammary buds. It has been used in the treatment of rat heart pieces during the isolation of mitochondria from rat heart. It has also been used for the iso |
| Biochem/physiol Actions | Dispase II is a neutral protease that hydrolyzes the N-terminal peptide bonds of non-polar amino acid residues. It may be used for separating many tissues and cells grown in vitro. The enzyme is very gentle and does not damage cell membranes. |
| Physical form | lyophilized powder containing calcium acetate and milk sugar |
| Preparation Note | A 50 U/mL stock solution may be prepared by dissolving the powder in a buffer containing 10 mM NaAc (pH 7.5) and 5 mM CaAc. It should be stored at 4 °C. |
| Unit Definition | One unit will hydrolyze casein to produce color equivalent to 1.0 mumole (181 mug) of tyrosine per min at pH 7.5 at 37°C (color by Folin-Ciocalteu reagent), unless otherwise indicated. |
性質(Properties)：
| activity | ≥0.5 units/mg solid |
| solubility | 10 mM NaAc (pH 7.5) and 5 mM CaAc: soluble |
| storage temp. | 2-8°C |
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