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當前位置：> 供求商機> 410201-5ML——環氧活化磁性瓊脂糖凝膠4HF
Product Introduction:
Epoxy-activated SepFast MAG is a magnetic agarose resin that can be readily employed to make various magnetic affinity chromatography adsorbents for both small scale and large scale purification applications.
Epoxy-activated agarose can be used for the immobilization of sugars and other carbohydrates via stable ether linkages with hydroxyl groups. It can also be used for the coupling of primary aminecontaining or thiol-containing molecules. Coupling biospecific ligands to Epoxy-activated agarose is a successful and well-documented technique. The reaction is very easy to conduct. No toxic chemical or special equipment is required. All the chemical bonds formed are very stable.
Epoxy activated SepFast is useful in coupling both small molecules and very large molecules. This pre-activated agarose base matrix can be readily employed to make various magnetic affinity chromatography media for both small scale and large scale purification applications.
Product Properties:
There is a choice of two different Epoxy activated magnetic resins that suits various ligands and applications.
• Epoxy activated SepFast MAG 4HF is made of highly cross-linked 4% agarose containing magnetic material. It shows high mechanical rigidity and allows harsher operational conditions, such as concentrated organic solvents, to be applied. It is useful for the coupling of protein molecules.
• Epoxy activated SepFast MAG 6HF is made of highly cross-linked 6% agarose containing magnetic material. It shows high mechanical rigidity and allows harsher operational conditions, such as concentrated organic solvents, to be applied. It has high levels of epoxy groups for applications that require high ligand densities. It is useful for the coupling of small molecules or peptides etc.
Agarose has long been used for chromatographic separations due to its excellent hydrophilic and low non-specific-binding nature. The particles have an open pore structure with excellent mass transfer properties to various molecules. The material is activated through attaching a hydrophilic carbon chain with epoxy group in the end.
| Group to be coupled | -NH2, -OH, -SH |
| Matrix | SepFast MAG 4HF: Highly cross-linked 4% magnetic agarose SepFast MAG 6HF: Highly cross-linked 6% magnetic agarose |
| Particle size* | 50 – 150 µm |
| Activation level | >30 µmol epoxy / ml for SepFast MAG 4HF >80 µmol epoxy / ml for SepFast MAG 6HF |
| pH stability | 4 -13 (ligand dependent) |
| Chemical stability | Compatible with all commonly used aqueous chemicals, provided the ligand to be coupled can withstand. Certain organic solvents could be used. |
| Storage | +4℃ - +8℃ |
Product Series:
| Product | Quantity | Code no. |
| Epoxy activated SepFast MAG 4HF | 5 ml | 410201-5ML |
| 50 ml | 410201-50ML | |
| 1 litre | 410201-1L | |
| Epoxy activated SepFast MAG 6HF | 5 ml | 410204-5ML |
| 50 ml | 410204-50ML | |
| 1 litre | 410204-1L |
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