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Biomatrica公司AssayStable PCRstable
閱讀：1569 發布時間：2018-3-27Biomatrica公司AssayStable PCRstable
Biomatrica授權蘇州螞蟻淘生物科技有限公司代理銷售旗下產品，
Biomatrica開發出一種新型的核心技術，旨在防止復雜的樣品和試劑在運輸、存儲以及處理過程中的降解，從而使科學家們能獲得更多重復性和可靠的實驗結果，提率，節省時間。Biomatrica目前的產品穩定核酸并防止降解，而無需冷藏
AssayStable® & PCRstable®
Precise positioning and controlled release of assay reagents not achievable by lyophilization
AssayStable and PCRstable are ambient temperature chemistries that allows assay manufacturers to precisely position, preserve, and release their assay reagents in any format using Biomatrica’s unique reagents with a simple air-dry process. PCRstable was developed for PCR-based assays, and AssayStable was developed for all other assays.
Overview
Assay Manufacturers:
Air-dry any assay reagent in-house and store at room temperature
No lyophilization and no low humidity chambers needed
Virtually any reagent stabilized by patented stabilizers
Assay Users:
Prepare any assay in a dry-stable format
Add template and go; no mixing needed
Rapid rehydration upon template addition
Use Cases
GenMark Diagnostics: Valve-Free Positioning and Preserving of Sample-to-Answer Diagnostic Devices
Thermo Fisher Ion AmpliSeq: Preservation of High Throughput Next Generation Sequencing Arrays
Focus Diagnostics: Enabling decentralized, point-of-care diagnostics through chemical biostabilization
Extending shelf-life of one-step, liquid RT-PCR assays for point of care
Instantaneous Preservation of Rare Cells in Blood to Evaluate Cancer Therapy Selection
PCR Assay Mastermix Ambient Stabilization Without Lyophilization
Stabilization of Multiplexed Panel for Next-Generation Sequencing
Product Data
Stabilization of qPCR assay
Included in dry-down:
- Taq
- Primers
- Reverse Transcriptase
- Dye (SYBR® Green)/Taqman®
- dNTPs
- Salts and Detergent
Stabilization of qPCR assay at ambient temperature
Figure 1: Components of qPCR assay, including Taq DNA polymerase, dNTPs, primers for RNase P, SYBR Green dye and PCR buffer were dried down in the presence of Biomatrica’s stabilizing formulation (Protected) or Non-Protected, and stored under high stress conditions at 45°C. At determined time points, samples were rehydrated with water and MgCl2 with the specified amount of genomic DNA template. Real time PCR amplification of a RNase P fragment was performed on the ABI 7300 Real-Time PCR System, using the equivalent amount of DNA with qPCR reagents stored at -20°C as a positive control. Data shown is for samples stored for 6 months at 45°C, corresponding to 2 years of ambient temperature storage, based on accelerated aging.
Stabilization of RT-qPCR assay at ambient temperature
Figure 2: All components of a RT-qPCR assay, including Taq DNA polymerase with reverse transcriptase activity, dNTPs, aptamer and PCR buffer including metal co-factor were dried down in the presence of Biomatrica’s stabilizing formulation (Protected) or Non-Protected, and stored under high stress conditions at 45°C. At determined time points, samples were rehydrated with water, primers and TaqMan probes, and a known input of RNA template was added. Reverse transcription and real time PCR amplification were performed on ABI 7300 Real-Time PCR System, using the equivalent amount of RNA samples with RT-qPCR reagents stored at -20°C as a positive control. Data shown is for samples stored for 3 weeks at 45°C, corresponding to 3 months of ambient temperature storage, based on accelerated aging.
qPCR Stabilized
Figure 3: Taq + dNTPs stabilized for 4 years at room temperature(based on accelerated aging).
Stabilization of a Limiting Quantity of Reverse Transcriptase
Figure 4: Stabilization of a limiting amount of reverse transcriptase for rapid screening ability.
Ambient Shipping: Stress Testing
Figure 5: Biomatrica stabilizers protected all samples, while non-protected shows complete degradation below 10 ng.