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AAT Bioquest公司熒光探針Rhod-4™使用說(shuō)明
蘇州螞蟻淘生物科技有限公司是AATBIO的中國(guó)大陸區(qū)授權(quán)代理商
美國(guó)AAT Bioquest Inc.(前身是ABD Bioquest，Inc.)是一家為從事生命科學(xué)研究、診斷研發(fā)及藥物開(kāi)發(fā)的科學(xué)家研發(fā)、生產(chǎn)和銷售生物分析研究試劑和試劑盒的公司。公司致力于光譜學(xué)檢測(cè)領(lǐng)域，包括吸收(顏色)、熒光和發(fā)光技術(shù)。AAT Bioquest的產(chǎn)品幫助*的科學(xué)家和生物醫(yī)藥研究者更好的了解生物化學(xué)、免疫學(xué)、細(xì)胞生物學(xué)和分子生物學(xué)等領(lǐng)域。AAT Bioquest會(huì)不斷研發(fā)新產(chǎn)品，快速地豐富各個(gè)領(lǐng)域的產(chǎn)品。
1）提供反應(yīng)探針和發(fā)光探針，生物素和端粒酶能夠應(yīng)用于標(biāo)記藥物小分子和生物聚合物，如蛋白、核酸以及其他碳水化合物；
2）研究并生產(chǎn)熒光和發(fā)光探針用于檢測(cè)蛋白，核酸和活細(xì)胞；
3）不斷推出新型的熒光和發(fā)光探針用于檢測(cè)多種酶，特別是檢測(cè)水解酶和氧化還原酶類；
4）致力于開(kāi)發(fā)用于信號(hào)轉(zhuǎn)導(dǎo)研究的試劑；
5）提供生理和神經(jīng)探針，特別是鈣離子指示劑和膜電位探針。
AAT Bioquest公司熒光探針Rhod-4™使用說(shuō)明
Calcium measurement is critical for numerous biological investigations. Fluorescent probes that show spectral responses upon binding Ca2+ have enabled researchers to investigate changes in intracellular free Ca2+ concentrations by using fluorescence microscopy, flow cytometry, fluorescence spectroscopy and fluorescence microplate readers. Rhod-2 is most commonly used among the red fluorescent calcium indicators. However, Rhod-2 AM is only moderately fluorescent in live cells upon esterase hydrolysis, and has very small cellular calcium responses. Rhod-4™ has been developed to improve Rhod-2 cell loading and calcium response while maintaining the spectral wavelength of Rhod-2. In CHO and HEK cells Rhod-4™ AM has cellular calcium response that is 10 times more sensitive than Rhod-2 AM. AAT Bioquest offers versatile packing sizes of Quest Rhod-4 to meet your special needs, e.g., 1 mg; 10x50 µg; 20x50 µg; HTS packages with no additional packaging charge
| Ex/Em (nm) | 524/551 |
| MW | 1015.96 |
| Kd (nM) | 451 |
| Solvent | DMSO |
| Storage | Freeze (<-15 °C) Minimize light exposure |
| Instruments | Fluorescence microplate reader, Fluorescence microscope, Flow cytometer |
| Category | GPCR Calcium GPCR Assays |
| Related | Calcium Channels
|
Calculators
Common stock solution preparation
Table 1. Volume of DMSO needed to reconstitute specific mass of Rhod-4™, AM to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.
| 0.1 mg | 0.5 mg | 1 mg | 5 mg | 10 mg | |
| 1 mM | 98.429 µL | 492.145 µL | 984.291 µL | 4.921 mL | 9.843 mL |
| 5 mM | 19.686 µL | 98.429 µL | 196.858 µL | 984.291 µL | 1.969 mL |
| 10 mM | 9.843 µL | 49.215 µL | 98.429 µL | 492.145 µL | 984.291 µL |
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