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產品簡介
Jackson|二抗715-167-003*
通過木瓜蛋白酶消化完整IgG抗體產生Fab片段抗體，以去除整個Fc部分，包括鉸鏈區。 這些抗體是單價的，僅含有單個抗原結合位點。
通過木瓜蛋白酶消化完整IgG抗體產生Fab片段抗體，以去除整個Fc部分，包括鉸鏈區。 這些抗體是單價的，僅含有單個抗原結合位點。
詳細介紹
Jackson|二抗715-167-003*
通過木瓜蛋白酶消化完整IgG抗體產生Fab片段抗體，以去除整個Fc部分，包括鉸鏈區。 這些抗體是單價的，僅含有單個抗原結合位點。 Fab片段的分子量為約50kDa。
基于抗原結合測定和/或ELISA，抗體與全分子小鼠IgG反應。 它還與其他小鼠免疫球蛋白的輕鏈反應。 未檢測到針對非免疫球蛋白血清蛋白的抗體。 抗體可以與來自其他物種的免疫球蛋白交叉反應。
Jackson|二抗715-167-003*
Physical State: Freeze-dried solid
Storage:
Storage and Rehydration: Store freeze-dried solid at 2-8°C. Rehydrate with the indicated volume of dH2O and centrifuge if not clear. Prepare working dilution on day of use. Product is stable for about 6 weeks at 2-8°C as an undiluted liquid.
Extended Storage after Rehydration: Aliquot and freeze at -70°C or below. Avoid repeated freezing and thawing. Alternatively, add an equal volume of glycerol (ACS grade or better) for a final concentration of 50%, and store at -20°C as a liquid.
Expiration date: one year from date of rehydration. The expiration date may be extended if test results are acceptable for the intended use.
Purity: The antibody was purified from antisera by a combination of papain digestion and immunoaffinity chromatography using antigens coupled to agarose beads. Fc fragments and whole IgG molecules have been removed.
Buffer: 0.01M Sodium Phosphate, 0.25M NaCl, pH 7.6
Stabilizer: 15 mg/ml Bovine Serum Albumin (IgG-Free, Protease-Free)
Preservative: 0.05% Sodium Azide
Suggested Working Concentration or Dilution Range:
1:100 - 1:800 for most applications
Dilution factors are presented in the form of a range because the optimal dilution is a function of many factors, such as antigen density, permeability, etc. The actual dilution used must be determined empirically.