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AAT Bioquest公司熒光探針Rhod-4™使用說明;鈣的測量對于許多生物學研究至關重要。 在結合Ca2 +時顯示光譜響應的熒光探針使研究人員能夠通過熒光顯微鏡研究細胞內游離Ca2 +濃度的變化，......
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AAT Bioquest公司熒光探針Rhod-4™使用說明
蘇州螞蟻淘生物科技有限公司是AATBIO的中國大陸區*代理商
美國AAT Bioquest Inc.(前身是ABD Bioquest，Inc.)是一家為從事生命科學研究、診斷研發及藥物開發的科學家研發、生產和銷售生物分析研究試劑和試劑盒的公司。公司致力于光譜學檢測領域，包括吸收(顏色)、熒光和發光技術。AAT Bioquest的產品幫助*的科學家和生物醫藥研究者更好的了解生物化學、免疫學、細胞生物學和分子生物學等領域。AAT Bioquest會不斷研發新產品，快速地豐富各個領域的產品。
1）提供反應探針和發光探針，生物素和端粒酶能夠應用于標記藥物小分子和生物聚合物，如蛋白、核酸以及其他碳水化合物；
2）研究并生產熒光和發光探針用于檢測蛋白，核酸和活細胞；
3）不斷推出新型的熒光和發光探針用于檢測多種酶，特別是檢測水解酶和氧化還原酶類；
4）致力于開發用于信號轉導研究的試劑；
5）提供生理和神經探針，特別是鈣離子指示劑和膜電位探針。
AAT Bioquest公司熒光探針Rhod-4™使用說明
Calcium measurement is critical for numerous biological investigations. Fluorescent probes that show spectral responses upon binding Ca2+ have enabled researchers to investigate changes in intracellular free Ca2+ concentrations by using fluorescence microscopy, flow cytometry, fluorescence spectroscopy and fluorescence microplate readers. Rhod-2 is most commonly used among the red fluorescent calcium indicators. However, Rhod-2 AM is only moderately fluorescent in live cells upon esterase hydrolysis, and has very small cellular calcium responses. Rhod-4™ has been developed to improve Rhod-2 cell loading and calcium response while maintaining the spectral wavelength of Rhod-2. In CHO and HEK cells Rhod-4™ AM has cellular calcium response that is 10 times more sensitive than Rhod-2 AM. AAT Bioquest offers versatile packing sizes of Quest Rhod-4 to meet your special needs, e.g., 1 mg; 10x50 µg; 20x50 µg; HTS packages with no additional packaging charge
| Ex/Em (nm) | 524/551 |
| MW | 1015.96 |
| Kd (nM) | 451 |
| Solvent | DMSO |
| Storage | Freeze (<-15 °C) Minimize light exposure |
| Instruments | Fluorescence microplate reader, Fluorescence microscope, Flow cytometer |
| Category | GPCR Calcium GPCR Assays |
| Related | Calcium Channels
|
Calculators
Common stock solution preparation
Table 1. Volume of DMSO needed to reconstitute specific mass of Rhod-4™, AM to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.
| 0.1 mg | 0.5 mg | 1 mg | 5 mg | 10 mg | |
| 1 mM | 98.429 µL | 492.145 µL | 984.291 µL | 4.921 mL | 9.843 mL |
| 5 mM | 19.686 µL | 98.429 µL | 196.858 µL | 984.291 µL | 1.969 mL |
| 10 mM | 9.843 µL | 49.215 µL | 98.429 µL | 492.145 µL | 984.291 µL |