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Source

Mouse

Applications

WB,IP,IF

Reactivity

Species independent

Dilution

WB: 1:5000; IP: 1:200; IF: 1:1000

Storage

-20°C/1 year

Specificity

The antibody detects C-terminal, internal, and N-terminal V5-tag fusion proteins.

Source/Purification

The antibody was affinity-purified from mouse ascites by affinity-chromatography using specific immunogen.

Immunogen

Synthetic Peptide of V5-Tag

Form

PBS, pH 7.4, containing 0.5%BSA, 0.02% sodium azide as Preservative and 50% Glycerol.

Clonality

Monoclonal

Background

The V5 epitope tag is derived from a small epitope (Pk) present on the P and V proteins of the paramyxovirus of simian virus 5 (SV5). The V5 tag antibody can be helpful in detecting the recombinant proteins, some of which include transmembrane and secreted proteins fusion protein. This V5-tag antibody is prepared by immunizing synthetic peptide GKPIPNPLLGLDST coupled to KLH.

Concentration

1 mg/ml