詳細介紹
硫代-青色素3.5炔烴 Sulfo-CY3.5 alkyne
磺化Cy3.5(sulfo Cyanine 3.5; sulfo Cy3.5)是一種發紅色熒光的花青素類熒光染料。磺化Cy3.5是磺化修飾過的Cy3.5,水溶性好,它在顯影時可以用561nm或者594nm的激光束激發然后用 Texas Red? 或相似的濾片觀察,所以也是一種非常常用的標記染料。磺化Cy3.5可以用來標記蛋白,抗體,多肽,核酸分子,納米粒等。
磺化Cy3.5水溶性非常好,可在水相標記反應體系中直接使用,不需要有機溶劑助溶,特別適合標記對有機溶劑敏感的生物分子。另外由于強水溶性加染料分子本身帶電荷,磺化Cy3.5標記到生物分子表面后不會引發疏水性聚集,提高熒光標記產物的穩定性。
炔烴(alkyne)是常用生物標記基團。通過銅催化點擊化學反應(Copper-catalyzed azide–alkyne cycloaddition, CuAAC),炔烴可以和疊氮(azide)生成穩定的生物鏈接(bioconjugate)。點擊化學反應效率很高,可在水相中(也可有機相)快速完成,即便在低濃度(μM)下。點擊化學反應技術發展很成熟,各種相關試劑商業易得,各種生物標記應用。
Cy3.5標記后的產物Ex/Em是591nm/604nm。和它具有相似熒光特性的染料包括Texas Red?,Alexa Fluor 594, BODIPY? TR, DyLight 594等。
英文名稱:Sulfo-Cyanine3.5 alkyne
Sulfo-CY3.5 alkyne
中文名稱:硫代-青色素3.5炔烴
水溶性Cyanine3.5炔基
分子式:C41H40N3K3O13S4
分子量:1028.32
溶解性:易水溶,亦可溶于DMF, DMSO
質量控制:純度>95%
儲存條件:在-20°C中24個月。
運 輸:室溫下長達3周。避免長時間接觸光線。
結構式:
我公司其它染料:
ROX NHS ester, pure 6- isomer
BDP FL NHS ester
BDP TMR NHS ester
Cyanine3 NHS ester
Cyanine3.5 NHS ester
Cyanine5 NHS ester
Cyanine5.5 NHS ester
Cyanine7 NHS ester
Cyanine7.5 NHS ester
Pyrenebutyric acid NHS ester
Sulfo-Cyanine3 NHS ester
Sulfo-Cyanine5 NHS ester
Sulfo-Cyanine7 NHS ester
Dye azides
BDP FL azide
Coumarin 343 azide
Cyanine3 azide
Cyanine3.5 azide
Cyanine5 azide
Cyanine5.5 azide
Cyanine7 azide
Cyanine7.5 azide
FAM azide, 5-isomer
FAM azide, 6-isomer
PEP azide
Perylene azide
Pyrene azide 1
Pyrene azide 2
Pyrene azide 3
R110 azide, 5- isomer
R110 azide, 6- isomer
ROX azide, 5- isomer
Sulfo-Cyanine3 azide
Sulfo-Cyanine5 azide
Sulfo-Cyanine7 azide
TAMRA azide, 5-isomer
Dye alkynes
1-Ethynyl pyrene
3-Ethynyl perylene
BDP FL alkyne
Cyanine3 alkyne
Cyanine5 alkyne
Cyanine5.5 alkyne
Cyanine7 alkyne
Cyanine7.5 alkyne
FAM alkyne, 5-isomer
FAM alkyne, 6-isomer
Sulfo-Cyanine3 alkyne
Sulfo-Cyanine5 alkyne
TAMRA alkyne, 5-isomer
Dye maleimides
BDP FL maleimide
Cyanine3 maleimide
Cyanine5 maleimide
Cyanine5.5 maleimide
Cyanine7 maleimide
Cyanine7.5 maleimide
Sulfo-Cyanine3 maleimide
Sulfo-Cyanine5 maleimide
Dye hydrazides
Cyanine3 hydrazide
Cyanine5 Boc-hydrazide
Cyanine5 hydrazide
Cyanine5.5 hydrazide
Cyanine7 hydrazide
Cyanine7.5 hydrazide
Carboxylic acids
Cyanine3 carboxylic acid
Cyanine3.5 carboxylic acid
Cyanine5 carboxylic acid
Cyanine5.5 carboxylic acid
Cyanine7 carboxylic acid
Cyanine7.5 carboxylic acid
Pyrenebutyric acid
Sulfo-Cyanine3 carboxylic acid
Sulfo-Cyanine5 carboxylic acid
Sulfo-Cyanine7 carboxylic acid
Amino dyes
Cyanine3 amine
Cyanine5 amine
Cyanine5.5 amine
Cyanine7 amine
Cyanine7.5 amine
Sulfo-Cyanine7 amine