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BPS Bioscience Inc.
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AAV8 SaCas9

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品       牌BPS Bioscience

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更新時間:2023-10-10 23:07:54瀏覽次數:148次

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保存條件:-80°C(dryice)保質期:3-18month英文名:AAV8SaCas9數量:1供應商:BPSBioscienceInc
  • 保存條件:

    -80°C (dry ice)

  • 保質期:

    3-18 month

  • 英文名:

    AAV8 SaCas9

  • 數量:

    1

  • 供應商:

    BPS Bioscience Inc.

  • CAS號:

    /

  • 規格:

    50 µl x 2

Adeno-Associated Virus serotype 8 (AAV8) was isolated from rhesus monkey tissue, and the AAV8 rep and cap nucleotide sequences have 88% homology with AAV7 and 82% with AAV2. AAV8 exhibits greater transduction efficiency in the liver than other AAV serotypes. AAV8 and 9 have recently been used to correct disease-causing mutations and improve muscle function in mouse models of Duchenne muscular dystrophy.Cas9 is an endonuclease enzyme that introduces a double stranded break into the DNA when recruited to a specific DNA sequence by the sgRNA (single guide RNA). This double stranded break is repaired in mammalian cells either through Non-Homologous End Joining or Homologous Recombination. Non-Homologous End Joining often results in the deletion or insertion of several base pairs at the cut site, which, when resulting in a frameshift, causes the functional inactivation of the gene.SaCas9 (Staphylococcus aureus CRISPR-associated protein 9) has demonstrated high cutting efficiency in mammalian cells, and its smaller size makes it ideal for packaging into AAV. SaCas9 recognizes a longer protospacer adjacent motif (PAM) site, 5'-NNGRRT-3', than the more traditional SpCas9 (Streptococcus pyogenes CRISPR-associated protein 9). These AAV particles constitutively express SaCas9 under the control of a CMV promoter.
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