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當(dāng)前位置:BPS Bioscience Inc.>>PDE Assay Kits>> 60707PDE4D2 TR-FRET Assay Kit
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產(chǎn)品型號(hào)60707
品 牌BPS Bioscience
廠商性質(zhì)生產(chǎn)商
所 在 地
更新時(shí)間:2023-11-11 22:50:49瀏覽次數(shù):135次
聯(lián)系我時(shí),請(qǐng)告知來自 化工儀器網(wǎng)Phosphodiesterases (PDEs) play an important role in dynamic regulation of cAMP and cGMP signaling. PDE4D is a regulator of airway smooth-muscle contractility, and has been identified as a potential risk predictor for ischemic stroke. Additionally, PDE4D has been associated with asthma pathophysiology and bone formation. The PDE4D gene encodes at least 9 different isoforms.
The PDE4D2 TR-FRET Assay Kit is designed for identification of inhibitors of PDE4D2 using TR-FRET (Time Resolved Fluorescence Resonance Energy Transfer) technology. The assay is based on the generation of FAM-labeled nucleotide monophosphates by the phosphodiesterase. These phosphate groups bind to terbium-labeled nanoparticles, resulting in energy transfer from the terbium to the FAM, which emits a fluorescent signal at 520 nm. The change in fluorescent intensity can be easily measured using a fluorescence plate reader.
Using this kit, only two simple steps on a microtiter plate are required for the PDE4D2 activity assay. First, the fluorescent-labeled cAMP is incubated with a sample containing PDE4D2 for 1 hour. Second, a binding agent and a terbium donor are added to the reaction mix and incubated for 1 hour. Then, fluorescence intensity can be measured using a fluorescence reader.
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