The TIGIT:CD112 Homogeneous Assay Kit is designed to measure the inhibition of human T-cell immunoreceptor with Ig and ITIM domains (TIGIT) binding to CD112 (PVRL2/Nectin-2). The TIGIT:CD112 Homogeneous Assay Kit comes in a convenient AlphaLISA® format with purified biotinylated TIGIT, His-tagged CD112, and assay buffer to perform a total of 384 reactions. With this kit, only three simple steps on a microtiter plate are required. First, a sample containing TIGIT and an inhibitor of choice is incubated with the CD112 for 60 minutes. Next, acceptor beads are added, then donor beads, followed by reading the Alpha-counts.