The TET2 (Tet Methylcytosine dioxygenase 2) Homogeneous Assay Kit is designed to measure the activity of TET2 for screening and profiling applications. The TET2 Homogeneous Assay Kit comes in a convenient AlphaLISA® format, with enough biotinylated TET2 substrate, TET2 assay buffer, and purified TET2 for 384 reactions.

The key to the TET2 Homogeneous Assay Kit is a highly specific antibody that recognizes the hydroxymethylated substrate, and the increase in Alpha-counts is proportional to the hydroxymethylation of the substrate. First, a sample containing TET2 enzyme is incubated with the reaction mixture. Next, acceptor beads are added, followed by donor beads and reading the Alpha-counts.