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當(dāng)前位置：BPS Bioscience Inc.>>Ubiquitination>> 78820CBL-B-Driven SRC Ubiquitination Intrachain TR-FRET Assay Kit
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產(chǎn)品型號78820
品 牌BPS Bioscience
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更新時間：2023-11-12 23:00:28瀏覽次數(shù)：147次
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1,10-Phenanthroline, Monohydrate
A20, His tag, FLAG-tag (Sf9-derived
The CBL-B-driven SRC ubiquitination intrachain TR-FRET Assay Kit utilizes a Europium cryptate-labeled Ubiquitin Donor and a Cy5-labeled Ubiquitin Acceptor to complete the TR-FRET pairing. Since both the TR-FRET donor and acceptor are incorporated into poly-ubiquitin chains, this assay does not detect mono-ubiquitination. The FRET-based format requires no time-consuming washing steps, making it especially suitable for HTS applications as well as real-time analyses of polyubiquitination.
Figure 1: CBL-B-driven SRC ubiquitination intrachain TR-FRET Assay Kit schematic.
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