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當(dāng)前位置:BPS Bioscience Inc.>>Ubiquitination>> 79575CBL-B TR-FRET Assay Kit
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產(chǎn)品型號79575
品 牌BPS Bioscience
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更新時間:2023-11-12 23:06:26瀏覽次數(shù):153次
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A20, His tag, FLAG-tag (Sf9-derived
Human Casitas B-lineage lymphoma proto-oncogene b (CBL-B) is an E3 ubiquitin-protein ligase that functions as a negative regulator of T-cell activation. It is a promising drug target in cancer immunotherapy. The CBL-B TR-FRET Assay Kit is designed to measure CBL-B auto-ubiquitination activity in a homogeneous 384 reaction format. It utilizes biotin-labeled Ubiquitin and a terbium-labeled antibody recognizing the GST-tagged CBL-B protein to complete the TR-FRET pairing. This FRET-based assay requires no time-consuming washing steps, making it especially suitable for high throughput screening applications.
Figure: Illustration of Assay Principle
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