產(chǎn)品名稱:Invitrogen 18009019 CALF INTESTINAL ALK. PHOSPH.
貨 號(hào):18009019
規(guī) 格:1000 units
分 類:DNA/RNA 修飾酶
品 牌:Invitrogen
國產(chǎn)/進(jìn)口:進(jìn) 口
儲(chǔ)存溫度:-20°C
特惠價(jià)格:致 電
庫存狀態(tài):現(xiàn) 貨
銷售商:上海慧穎生物科技有限公司
慧穎生物專業(yè)供應(yīng)Invitrogen 18009019 CALF INTESTINAL ALK. PHOSPH.常備現(xiàn)貨,*。主營Life Invitrogen產(chǎn)品,囊括TRlzol ?Reagent、TRIZOL LS Reagent 、B-27添加劑、凝膠填料、常規(guī)化學(xué)試劑、培養(yǎng)基及原料試劑、各類胎牛血清、馬血清等,如有需要,詳詢。
描述:
Calf Intestinal Alkaline Phosphatase (CIAP) is a phosphomonoesterase that removes 3′ and 5′ phosphates from DNA and RNA.
Concentration: 20 units/μL
Applications: Dephosphorylation of 5′-phosphorylated termini of vector DNA to prevent self-ligation (1). Dephosphorylation of 5′ termini of nucleic acids prior to forward reaction with kinase.
Source: Purified from calf intestinal mucosa.
Performance and Quality Testing: Endodeoxyribonuclease, 3′ exodeoxyribonuclease, and ribonuclease assays; dephosphorylation efficiency measured in a transformation assay.
Unit Definition: One unit hydrolyzes 1 μmol of 4-nitrophenyl phosphate in 1 min. at 37°C.
Unit Reaction Conditions: 1 M diethanolamine buffer, 10 mM 4-nitrophenyl phosphate, 0.25 mM MgCl2 (pH 9.8) in 900 μl for 10 min. at 37°C.
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