13
產品名稱：Invitrogen 15224-025 T4 DNA 連接酶
貨 號：15224-025
規 格：500U(1U/ul)
分 類： DNA/RNA 修飾酶
品 牌：Invitrogen
國產/進口：進 口
儲存溫度：-20°C
特惠價格：致 電
庫存狀態：現 貨
銷售商：上海慧穎生物科技有限公司
慧穎生物專業供應Invitrogen 15224-025 T4 DNA 連接酶，常備現貨，*。主營Life Invitrogen產品，囊括TRlzol ?Reagent、TRIZOL LS Reagent 、B-27添加劑、凝膠填料、常規化學試劑、培養基及原料試劑、各類胎牛血清、馬血清等，如有需要，詳詢。
描述：
T4 DNA Ligase catalyzes the formation of phosphodiester bonds in the presence of ATP between double-stranded DNAs with 3′ hydroxyl and 5′ phosphate termini. The unique T4 DNA Ligase buffer optimizes ligation, which can be performed in 5 minutes. Single-stranded nucleic acids are not substrates for this enzyme.
Applications
Cloning (blunt-end or cohesive-end ligation) and adding linkers or adapters to blunt-ended DNA
Source
Purified from E. coli lambda lysogen NM989
Performance and quality testing
Endodeoxyribonuclease, 3′ and 5′ exodeoxyribonuclease assays; ligation efficiency tested
Unit definition
One unit catalyzes the exchange of 1 nmol 32P-labeled pyrophosphate into ATP in 20 min at 37°C. One unit is equal to approximay 300 cohesive-end ligation units.
Unit reaction conditions
66 mM Tris-HCl (pH 7.6), 6.6 mM MgCl2, 10 mM DTT, 66 μM ATP, 3.3 μM 32P-labeled pyrophosphate, and enzyme in 0.1 mL for 20 min at 37°C.
同類產品主要有:
| 貨號 | 名稱 | 規格 |
| 14750100 | BLOCKIT ALEXA FLUOR RED OLIGO | 20 UM- 250UL |
| K494500 | BLOCK IT U6 ENTRY VECTOR KIT | 20 RXNS |
| 15630080 | HEPES | 10ml |
| NP0060 | NUPAGE MES BIS-TRIS BFR KIT | COMBO |
| NP0321BOX | NuPAGE? Novex 4-12% Bis-Tris Gel 1.0 mm, 10 Well | 10 gels?box |
| 12355036 | PFX50 DNA POL., 500RXN | 500 RXNS |
| A11180 | CLONEMINER CDNA LIB KIT, VER2 | 5 RXN KIT |
| L150201 | GENERACER SUPERSCRPT TA | 5 CDNA- 50 PCR RXNS |
| 11791020 | LR CLONASE II ENZYME MIX | 20 REACTIONS |
| 10928-034 | 含有Platinum? Taq DNA聚合酶的SuperScriptTM II一步法RT-PCR系統 | 25 rxns |
| 10928-042 | 100 rxns | |
| 11904-018 | 用于RT-PCR的SuperScriptTM II*鏈合成系統 | 50 rxns |
| 18080-200 | SuperScriptTM III CellsDirect cDNA合成試劑盒 | 25 rxns |
| 18080-300 | 100 rxns | |
| 18080-400 | SuperScriptTM III*鏈合成SuperMix | 50 rxns |
| 18068-015 | DNase I擴增級 | 100U(1U/ul) |
| 48190-011 | 隨機引物 | 9 units |
| K210005 | PURELINK PLASMID MIDI 50 | 50 REACTIONS |
| S7563 | SYBR? Green I核酸凝膠染料 | 500ul |
| S7567 | 1ml | |
| S7564 | SYBR? Green II RNA凝膠染料 | 500ul |
| S7568 | 1ml | |
| 11811031 | GENETICIN | 5G |
| 10459014 | DMRIE-C REAGENT | 1ml |
| 12309019 | OPTI PRO SFM | 1000ml |
| 11514015 | PLUS REAGENT | 0.85 ML |
| 12651014 | FREESTYLE CHO EXPRESSION MEDIU | 1000ML |
| 12338018 | FREESTYLE 293 EXPRESSION MED | 1000ML |
| 12347019 | 293FECTIN | 1ml |
| 16447100 | FreeStyle MAX | 1 ml |
| R25001 | ZEOCIN | 1g |
| 15224-017 | T4 DNA 連接酶 | 100U(1U/ul) |
| 15224-025 | 500U(1U/ul) | |
| 15224-090 | 4x500U(1U/ul) | |
| 15224-041 | 250U(5U/ul) | |
| 18047-019 | DNase I | 20,000U |
| 18068-015 | DNase 擴增級 | 100U |
| 18021-014 | 核糖核酸酶H | 30U(2U/ul) |
| 18021-071 | 4x30U(2U/ul) | |
| 18162-016 | DNA聚合酶I/DNase I | 250U |
| 18005-017 | T4 DNA 聚合酶 | 50U(5U/ul) |
| 18005-025 | 250U(5U/ul) | |
| 25530-015 | 蛋白酶K | 100mg |
| 25530-031 | 1g | |
| 18374-058 | 5`RACE系統， | 10 rxns |
| 18373-019 | 3`RACE系統， | 20 rxns |
| MPK10096 | Neon? Transfection System 100 μL Kit | |
| K280020 | ZERO BLUNT TOPO PCRCLON KIT | 20 RXN |
| 18012021 | LARGE FRAGMENT | 100 UN |
| 18021014 | RNASE H (E.COLI) | 30 UN |
| 4311816 | AmpliTaq Gold? DNA Polymerase, 1000 units with Gold Buffer and MgCl2 solution | 1000 un |
| 18092015 | CDNA SIZE FRAC COLUMNS | 3 COLUMNS |
| 450512 | BLOCK-IT H1 RNAI VECTOR KIT | 20 RXNS |
| 10511012 | 1 kb DNA Extension Ladder | 100 UG |
Invitrogen產品種類較多，本展臺無法一一體現，以上是部分產品，若有需要的產品，請及時與我們取得，咨詢。
電 話：021-61277397/56980388，
傳 真：
Q Q： 1171206955
郵 箱：1242635944
網 址：http://www.biohysw。。ｃｏｍ/
