美國CYTOO細胞微納米結構圖案多孔板和芯片與xonamicrofluidics品牌微流控細胞微圖案化培養和分化、相互作用資料

美國CYTOO細胞微納米結構圖案多孔板和芯片應用文獻
xonamicrofluidics品牌微流控細胞微圖案化產品應用文獻

基底材料的拓撲形貌是影響細胞行為的重要因素之一,細胞與微納米結構圖案表面相互作用研究在細胞生物學、再生醫學和組織工程領域具有重要的意義。材料表面微納米圖案化不但可以提供結構模版,用以研究細胞對生長環境的響應特性,而且可以為組織再生用支架和植入性器件的設計提供基礎數據。

實驗結果顯示,細胞會根據材料基底表面的圖案結構產生相應的形變,而且這種形變響應與圖案幾何形狀、尺寸,細胞伸展和基底表面的黏附蛋白濃度有關。在一定范圍內,圖案越大、越深,細胞形變越大；細胞伸展面積越大,形變越小：黏附蛋白可以促進細胞伸展,從而使細胞形變減小.微米坑圖案能夠明顯促進細胞在材料表面的黏附；微米溝槽圖案 則對細胞具有接觸誘導效應,可以誘導細胞響應所生長的微納米溝槽結構進行骨架重排,從而引起細胞取向行為變化.

CYTOO品牌的細胞微圖案產品包括CYTOOPLATE?細胞微圖案微孔培養板和CYTOOChips細胞微圖案芯片:

CYTOOPLATE?細胞微圖案微孔培養板是一個高清晰度的低熒光硼硅玻璃底細胞光刻微圖案， CYTOOChips細胞微圖案芯片

可以控制細胞粘附性狀、大小、細胞培養分化等，研究細胞取，這種方法可以得到穩定的具有顯著細胞黏附反差的 "微圖案化表面. 利用該圖案化表面技術引導細胞黏附后的取向，先在細胞無法貼附的Cytophobic 涂料處理，再以光蝕刻 "的技術，精準地留下特定的幾何形狀，并在這樣的幾形狀上涂上特定的細胞間質蛋白(extracellular " matrix , ECM, such as fibronecin, collgene, Laminin, ect…) 。這樣的細胞培養工具借由精準的控制細胞貼附的型式，提供了體外培養細胞結構上的引導，使細胞表現出更接近體內生理的表型及框架，這樣的工具可以:
◆提高試驗的再現性，靈敏性和歸納量化復雜的生物性狀
◆進一步分析細胞結構機械性刺激和細胞功能之間的關系
◆模擬重現細胞在生物體內的環境，并使性狀易于觀察分析

"CYTOO 細胞微圖案(CYTOO micropattern)使體外培養細胞重現體內應有的細胞極性，有組織的整齊排列，細胞移動的限制!

CYTOO 細胞粘附微圖案

量化活細胞分析的突破
精準的對照條件在CYTOO 陣列上，細胞呈現相同的結構

標準的培養皿中的細胞 CYTOO培養產品中的細胞
"細胞貼附在涂有細胞間質蛋白的微圖案(micropattern)陣列上時，會隨著微圖案（micropattern）的引導在不能貼附的材質上張開成特定的幾何結構。這樣特定形狀的貼附接觸分布，促使細胞產生固定且再現性*的極性。細胞位置、細胞形狀、細胞極性、以及胞器位置都可標準化.
重現體內細胞的物理環境
使體外培養細胞的生理性狀更接近體內組織

CYTOOchipTM細胞微圖案芯片
19.5x19.5 mm 蓋玻片，由光刻法在高質低熒光硼硅酸鹽玻璃上刻畫微圖形，用于倒置顯微鏡， "網狀結構打印在芯片底部，使用戶可以隨時找到所需細胞。這些芯片與CYTOOchamber芯片槽結合可實現高分辨率活細胞成像。
標準芯片和平板可用于所有一般用途，初始包的設計是用來開發微圖形形狀和尺寸的， Arena芯片分為不同尺寸的磁盤，客戶定制產品可以使您達到*實驗方案。

CYTOOchips細胞微圖案芯片客戶定制產品包括19.5x19.5 mm 蓋玻片，客戶可定制微圖形，且粘附蛋白可適用于多種應用和實驗。定制CYTOOchipszui小量為1組18個芯片，且包裹纖連蛋白或活性表面。
客戶定制范圍包括：

在我們的標準幾何圖形的基礎上，更大、更小、或密度不同
混合不同的微圖形形狀
調整芯片表面、面積或形狀，方便研究幾何圖形如何影響細胞結構和行為
平行放置不同微圖形
zui低線寬3微米，點5微米

產品規格

CYTOOCHIPS細胞微圖案芯片,一包18個
產品名稱	貨號	(PATTERN)圖案	SIZE規格	COATING 涂層
CYTOOCHIPS? MOTILITY A X18	10-031-00-18	Multiple	Multiple	Activated
CYTOOCHIPS? ARENA A X18	10-020-00-18	Disc	Multiple	Activated
CYTOOCHIPS? CUSTOM A X18	10-950-00-18	Multiple	Custom	Activated
CYTOOCHIPS? L-L-A X18	10-015-00-18	L	Large (1600 μm²)	Activated
CYTOOCHIPS? L-S-A X18	10-013-00-18	L	Small (700 μm²)	Activated
CYTOOCHIPS? L-M-A X18	10-014-00-18	L	Medium (1100 μm²)	Activated
CYTOOCHIPS? STARTER'S A X18	10-900-00-18	Multiple	Multiple	Activated
CYTOOCHIPS? DC-S-A X18	10-001-00-18	Disc	Small (700 μm²)	Activated
CYTOOCHIPS? DC-M-A X18	10-002-00-18	Disc	Medium (1100 μm²)	Activated
CYTOOCHIPS? DC-L-A X18	10-003-00-18	Disc	Large (1600 μm²)	Activated
CYTOOCHIPS? CW-L-A X18	10-006-00-18	Crossbow	Large (1600 μm²)	Activated
CYTOOCHIPS? CW-S-A X18	10-004-00-18	Crossbow	Small (700 μm²)	Activated
CYTOOCHIPS? CW-M-A X18	10-005-00-18	Crossbow	Medium (1100 μm²)	Activated
CYTOOCHIPS? Y-L-A X18	10-012-00-18	Y	Large (1600 μm²)	Activated
CYTOOCHIPS? Y-M-A X18	10-011-00-18	Y	Medium (1100 μm²)	Activated
CYTOOCHIPS? Y-S-A X18	10-010-00-18	Y	Small (700 μm²)	Activated
CYTOOCHIPS? H-L-A X18	10-009-00-18	H	Large (1600 μm²)	Activated
CYTOOCHIPS? H-M-A X18	10-008-00-18	H	Medium (1100 μm²)	Activated
CYTOOCHIPS? H-S-A X18	10-007-00-18	H	Small (700 μm²)	Activated

CYTOOplates細胞微圖案培養板

CYTOOplates平板為標準SBS微平板版式，由光刻法在高質低熒光硼硅酸鹽玻璃底部刻畫微圖形，可適用于高內涵篩選，且可適用于所有普通HCS成像平臺和實驗室自動化儀器。
"CYTOOplates客戶定制產品包括96孔標準SBS版式，客戶可定制微圖形，且粘附蛋白可適用于多種應用和實驗， "無論所需的微圖形形狀、尺寸或96孔布局如何。定制zui小量為10個平板，且包裹纖連蛋白或活性表面。
客戶定制范圍包括:

在我們的標準幾何圖形的基礎上，更大、更小、或密度不同
混合不同的微圖形形狀
調整平板表面、面積或形狀，方便研究幾何圖形如何影響細胞結構和行為
平行放置不同微圖形
zui低線寬3微米，點5微米

CYTOOPLATES細胞微圖案培養板訂貨信息,一包5塊
產品名稱	貨號	(PATTERN)圖案	SIZE規格	COATING涂層
CYTOOPLATES? 96 RW L-S-A	20-013-00	L	Small (700 μm²)	Activated
CYTOOPLATES? 96 RW L-L-A	20-015-00	L	Large (1600 μm²)	Activated
CYTOOPLATES? 96 RW L-M-A	20-015-00	L	Medium (1100 μm²)	Activated
CYTOOPLATES? 96 RW DC-S-A	20-001-00	Disc	Small (700 μm²)	Activated
CYTOOPLATES? 96 RW DC-M-A	20-002-00	Disc	Medium (1100 μm²)	Activated
CYTOOPLATES? 96 RW DC-L-A	20-003-00	Disc	Large (1600 μm²)	Activated
CYTOOPLATES? 96 RW CW-S-A	20-004-00	Crossbow	Small (700 μm²)	Activated
CYTOOPLATES? 96 RW CW-M-A	20-005-00	Crossbow	Medium (1100 μm²)	Activated
CYTOOPLATES? 96 RW CW-L-A	20-006-00	Crossbow	Large (1600 μm²)	Activated
CYTOOPLATES? 96 RW H-S-A	20-007-00	H	Small (700 μm²)	Activated
CYTOOPLATES? 96 RW H-M-A	20-008-00	H	Medium (1100 μm²)	Activated
CYTOOPLATES? 96 RW H-L-A	20-009-00	H	Large (1600 μm²)	Activated
CYTOOPLATES? 96 RW Y-S-A	20-010-00	Y	Small (700 μm²)	Activated
CYTOOPLATES? 96 RW Y-M-A	20-011-00	Y	Medium (1100 μm²)	Activated
CYTOOPLATES? 96 RW Y-L-A	20-012-00	Y	Large (1600 μm²)	Activated
CYTOOPLATES? 96 RW STARTER	20-900-00	Multiple	Multiple	Activated
CYTOOPLATES? 96 RW MOTILITY A	20-031-00	Line	Multiple	Activated

CYTOO chamber芯片槽
單孔芯片槽提供了大面積的成像區域，可承裝10,000個細胞圖形，這款產品尤其適合當您有超過4 "個不同的幾何圖形打印在芯片上的時候，以及像是有絲分裂的細胞的特例測量。次產品更適用于高分辨率活細胞成像。
4孔芯片槽可提供每孔上千種圖形在各情境下的平行免疫熒光實驗，Arena芯片和Motility芯片的4象限設計使之適用于4孔芯片槽。
優勢和主要特征:

可重復使用，高溫滅菌
磁性裝置使用簡單
35mm足跡
與普通顯微鏡臺兼容
zui多四平行

CYTOO chamber灌流槽
"閉合單通道或雙通道CYTOOchamber灌流槽用于活細胞成像,使用簡單,專門設計用于我們的CYTOOchips芯片. 主要部分為透明的丙烯酸材料，底板是黑色的陽極化處理鋁合金，部件還包括一圈硅墊。通道尺寸可根據要求定制。
優勢和主要特征：

- 閉合灌流槽
- 培養基補充簡易
- 與CYTOOchips兼容
- 磁性裝置使用簡單
- 可重復利用
- 單雙孔道標準配置，客戶定制通道尺寸
- 可配置顯微鏡臺適配器

Xonamicrofluidics品牌細胞微溝槽圖案流體培養板
貨號	產品名稱	圖片
RD150	Round Device (150 um microgroove barrier) Size: ~ 21 mm diameter Our round device version of the standard 150 um microgroove barrier has an approximate diameter of 21 mm, making it compatible with some 35 mm glass bottom dishes that many researchers use in live cell imaging systems. Glass bottom dishes used should have an inner aperture of 21 mm or more to accommodate the device. At least 22 mm is recommended in order to have room to position the device. Larger glass bottom dishes can be used, as well as glass cover slips, if desired.
RD450	Round Device (450 um microgroove barrier) Size: ~ 21 mm diameter Our round device version of the standard 450 um microgroove barrier has an approximate diameter of 21 mm, making it compatible with some 35 mm glass bottom dishes that many researchers use in live cell imaging systems. Glass bottom dishes used should have an inner aperture of 21 mm or more to accommodate the device. At least 22 mm is recommended in order to have room to position the device. Larger glass bottom "dishes can be used, as well as glass cover slips, if desired.
RD900	Round Device (900 um microgroove barrier) Size: ~ 21 mm diameter Our round device version of the standard 900 um microgroove barrier has an approximate diameter of 21 mm, making it compatible with some 35 mm glass bottom dishes that many researchers use in live cell imaging systems. Glass bottom dishes used should have an inner aperture of 21 mm or "more to accommodate the device. At least 22 mm is recommended in order to "have room to position the device. Larger glass bottom dishes can be used, as "well as glass cover slips, if desired.
SND150	Standard Neuron Device (150 um microgroove barrier) Size: ~ 22 mm – ~23mm The 150 um neuron device with its shorter microgroove barrier is suitable for researchers who would like to isolate both axons and dendrites. It is also suitable for certain "neuronal cultures that do not grow long processes. Researchers who wish to study the early events of axonal and dendritic growth "may also find the 150 um device useful. The 150 um device "also offers the benefits of fluidic isolation and culture organization for transport studies.
SND450	Standard Neuron Device (450 um microgroove barrier) Size: ~ 22 mm – ~23mm "The 450 um neuron device is our most popular device. For most "applications it is suitable for separating cell bodies from axons. After two weeks, dendrites in E18 rat cortical neurons have typically not crossed the microgroove barrier. "The 450 um neuron device also provides the added features of fluidic "isolation and culture organization for transport studies.
SND900	Standard Neuron Device (900 um microgroove barrier) Size: ~ 22 mm – ~23mm The 900 um neuron device, with its wide microgroove barrier, is suitable for researchers who would like to perform long term experiments while ensuring axonal isolation. "In some cultures, after two weeks of growth dendrites may cross the 450 um barrier thereby necessitating the use of the 900 um device.It is also suitable "for certain neuronal cultures which grow long processes. Researchers who "also wish to make sure they are isolating * axons only in the distal "chamber may also find the 900 um device useful. The 900 um device also offers the benefits of fluidic isolation and culture organization for transport studies.
TCND500	Triple Chamber Neuron Device (500 um microgroove barrier) Size: ~ 38 mm – ~24mm The TCND500 consists of (2) 500 um microgroove barriers with a 500 um central chamber. This device makes it possible to culture cortical "neurons in the left and right chambers while culturing hippocampal neurons, or other combinations of cells, in the middle. The triple chamber is also suitable for transport studies where the researcher wishes to treat the middle of the axon growing in the middle chamber while observing effects at the growth cone or in the somal compartment. Neurons can also be loaded in the central chamber and different treatments applied to the left and right compartments.
TCND1000	Triple Chamber Neuron Device (1000 um microgroove barrier) Size: Size: ~ 38 mm – ~24mm The TCND1000 consists of (2) 500 um microgroove barriers with a 1000 um central chamber. This device makes "it possible to culture cortical neurons in the left and "right chambers while culturing hippocampal neurons, or other combinations of cells, in the middle. The triple chamber is also suitable for transport studies where the researcher wishes to treat the middle of the axon growing in the middle chamber while observing effects at the growth cone or in the somal compartment. Neurons can also be loaded in the central chamber and different treatments "applied to the left and right compartments.
uLP	Microfluidic Local Perfusion Device barrier, (50 um microgroove 75 micron perfusion channel, 500 um microgroove barrier) Size: ~ 40 mm – ~24 mm "Our Microfluidic Local Perfusion Chamber was first published in Neuron. "2010 Apr 15;66(1):57-68. This device has a 75 micron perfusion "chamber with a set of microgrooves on the left 50 um in width and a set of "microgrooves on the right 500 um in width. This device, in conjuction with a "syringe pump or other suitable equipment, allows the researcher to perfuse "selected treatments and compounds across the target area. Syringe pump or "other suitable equipment required for proper use. For more indepth "information please see Neuron. 2010 Apr 15;66(1):57-68.