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Source

Rabbit

Applications

WB,ELISA

Reactivity

Human,Mouse,Rat

Dilution

WB: 1:500 - 2000; ELISA: 1:20000. Not yet tested in other applications.

Storage

-20°C/1 year

Specificity

Histone H3 Polyclonal Antibody detects endogenous levels of Histone H3 protein only when acetylation at K5.

Source/Purification

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Immunogen

Synthesized peptide derived from human Histone H3 around the non-acetylation site of K5.

Uniprot No

P68431/Q71DI3/P84243/Q6NXT2

Alternative names

Histone H3

Form

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Clonality

Polyclonal

Background

histone cluster 1 H3 family member a(HIST1H3A) Homo sapiens Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. This structure consists of approximately 146 bp of DNA wrapped around a nucleosome, an octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a replication-dependent histone that is a member of the histone H3 family. Transcripts from this gene lack polyA tails; instead, they contain a palindromic termination element. This gene is found in the large histone gene cluster on chromosome 6p22-p21.3. [provided by RefSeq, Aug 2015],

Mol.Wt (Da)

15404/15388/15328/15214

Concentration

1 mg/ml