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Source

Rabbit

Applications

WB,IHC,ELISA

Reactivity

Human

Dilution

WB: 1:500 - 1:2000. IHC: 1:100-300 ELISA: 1:20000. Not yet tested in other applications.

Storage

-20°C/1 year

Specificity

AFP Polyclonal Antibody detects endogenous levels of AFP protein.

Source/Purification

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Immunogen

The antiserum was produced against synthesized peptide derived from the Internal region of human AFP. AA range:371-420

Uniprot No

P02771

Alternative names

AFP; HPAFP; Alpha-fetoprotein; Alpha-1-fetoprotein; Alpha-fetoglobulin

Form

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Clonality

Polyclonal

Isotype

IgG

Background

alpha fetoprotein(AFP) Homo sapiens This gene encodes alpha-fetoprotein, a major plasma protein produced by the yolk sac and the liver during fetal life. Alpha-fetoprotein expression in adults is often associated with hepatoma or teratoma. However, hereditary persistance of alpha-fetoprotein may also be found in individuals with no obvious pathology. The protein is thought to be the fetal counterpart of serum albumin, and the alpha-fetoprotein and albumin genes are present in tandem in the same transcriptional orientation on chromosome 4. Alpha-fetoprotein is found in monomeric as well as dimeric and trimeric forms, and binds copper, nickel, fatty acids and bilirubin. The level of alpha-fetoprotein in amniotic fluid is used to measure renal loss of protein to screen for spina bifida and anencephaly. [provided by RefSeq, Jul 2008],

Other

AFP, Alpha-fetoprotein; Alpha-fetoprotein; Alpha-1-fetoprotein; Alpha-fetoglobulin

Mol.Wt (Da)

68678

Concentration

1 mg/ml