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Kerafast代理EB0010現(xiàn)貨Anti-VSV-G [8G5F11] Antibody操作使用

時間:2024-11-11閱讀:205

This monoclonal antibody reacts with VSV-G protein from the Indiana serotype.


Highlights:

*Shows reactivity against VSV-G pseudotyped lentiviruses (see references below)

*Suitable for Western Blot, Flow Cytometry and Immunofluorescence applications

*Fromthe hybridoma 8G5F11

Vesicular stomatitis virus (VSV) is a well studied, enveloped, negative-strand RNA virus. The VSV genome encodes for 5 proteins: N, P, M, G, and L. The G protein (glycoprotein) is located at the virion surface and is responsible for virus attachment and penetration. Additionally, many lentivrial vectors are pseudotyped with VSV-G from the Indiana serotype.


Product Type:Antibody
Name:Anti-VSV-G [8G5F11]
Antigen:VSV-G
Accession ID:P03522
Host:Mouse
Isotype:EB0010: IgG2a kappa
Recombinant versions: see product name
Clonality:Monoclonal
Clone Name:8G5FL11 (I1)
Specificity:VSV-Ind glycoprotein (G) protein
Reactivity:Human
Immunogen:VSV infection
Format:Liquid
Purification Method:Protein G purified
Buffer:EB0010: PBS, 0.05% (w/v) Sodium Azide
Recombinant versions: PBS with 0.02% Proclin 301
Tested Applications:Western blot (1:1000), flow cytometry, immunofluorescence
Concentration:1mg/mL
Amount:100uL
Storage:-20C (avoid repeated freeze / thaw cycles)
Shipped:Cold packs






Kerafast代理EB0010現(xiàn)貨Anti-VSV-G [8G5F11] Antibody操作使用

參考文獻(xiàn):

1. Gregory DA, Olinger GY, Lucas TM, Johnson MC. Diverse viral glycoproteins as well as CD4 co-package into the same human immunodeficiency virus (HIV-1) particles. Retrovirology. 2014 Apr 3;11(1):28.

2. Kubo S, Mitani K. A New Hybrid System Capable of Efficient Lentiviral Vector Production and Stable Gene Transfer Mediated by a Single Helper-Dependent Adenoviral Vector. JVirol 77: 2964-2971, 2003.

3. Kobayashi M, Iida A, Ueda Y, and Hasegawa M. Pseudotyped Lentivirus Vectors Derived from Simian Immunodeficiency Virus SIVagm with Envelope Glycoproteins from Paramyxovirus. JVirol 77: 2607-2614, 2003.

4. Burns JC, Friedmann T, Driever W, Burrascano M, Yee JK. Vesicular stomatitis virus G glycoprotein pseudotyped retroviral vectors: Concentration to very high titer and efficient gene transfer into mammalian and nonmammalian cells. PNAS 90: 8033-8037, 1993.

5. Vandepol SB, Lefrancois L, Holland JJ. Sequences of the Major Anitbody Binding Epitopes of the Indiana Serotype of Vesicular Stomatitis Virus. Virology 148: 312-325 (1986).

6. Lefrancios L, Lyles DS. The interaction of antibody with the major surface glycoprotein of vesicular stomatitis virus. I. Analysis of neutralizing epitopes with monoclonal antibodies. Virology 121: 157-167, 1982.


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