細(xì)胞免疫熒光主要用于蛋白定位研究,相互作用研究和細(xì)胞信號轉(zhuǎn)導(dǎo)研究。細(xì)胞免疫熒光就是將免疫學(xué)方法和熒光標(biāo)記技術(shù)相結(jié)合,研究特異性抗原在細(xì)胞內(nèi)的分布。細(xì)胞免疫熒光特性高,敏感性強(qiáng),速度快,主要原理也是利用抗原抗體反映抗原抗體結(jié)合后再用熒光標(biāo)記,通過顯微鏡下觀察來確定某種特異性抗原是否存在。
根據(jù)培養(yǎng)的細(xì)胞類型,可分為貼壁細(xì)胞和懸浮細(xì)胞(淋巴細(xì)胞,血液的白細(xì)胞)。貼壁細(xì)胞有天然貼壁的屬性,而懸浮細(xì)胞不依賴支持物表面,在培養(yǎng)液中呈懸浮狀態(tài)生長。
免疫熒光實驗的基礎(chǔ)前提就是讓細(xì)胞能固定在玻片上。懸浮細(xì)胞如何貼壁或者固定到玻片上,是科研人員面對的一個難題。傳統(tǒng)上,或用細(xì)胞離心甩片機(jī)制備細(xì)胞片或直接涂片法制備細(xì)胞涂片,然后把細(xì)胞片于乙醇或丙酮或多聚甲醛固定。無論甩片還是涂片,都是黑盒子實驗。每個人操作手法不一樣,即使同一個人操作,每一批實驗,也都沒法評價細(xì)胞片上的細(xì)胞固定的情況,而這一步至關(guān)重要。盲目而又沒底的只能硬著頭皮往下做。浪費的不僅是時間,還有抗體等很多試劑。如何解決這些痛點?懸浮細(xì)胞免疫熒光專用玻片Shi-Fix Coverslips帶來解決方案!
您是否希望懸浮細(xì)胞的免疫熒光像貼壁細(xì)胞一樣容易?現(xiàn)在就是這樣!
Shi-fix™玻片允許懸浮細(xì)胞分層或直接作為單層生長。簡單,無憂的實驗方案,無需離心即可將懸浮細(xì)胞固定到載玻片上。只需將細(xì)胞添加到載玻片上,等待30分鐘,用PBS洗滌未結(jié)合的細(xì)胞并繼續(xù)免疫染色(或繼續(xù)培養(yǎng)以獲得所需的細(xì)胞密度)。
這些玻片也可用于染色懸浮細(xì)胞以進(jìn)行顯微鏡檢查,或用于固定懸浮細(xì)胞以進(jìn)行共聚焦顯微鏡檢查。更重要的是,如果需要,您還可以在細(xì)胞附著在玻片上時刺激它們。您可以像貼壁細(xì)胞一樣處理非貼壁細(xì)胞!
Do you wish suspension cell Immunofluorescence was as easy as that of adherent cells?
Now it is! Our innovative Shi-fix™ cover-slips or multi well plates allow suspension cells to be layered on them or directly grown as a monolayer. Easy, hassle-free protocol and no spin columns needed for fixing suspension cells to slides. Just add your cells to the coverslips, wait for 30 mins, wash unbound cells with PBS and proceed to immunostaining.Or continue the culture to obtain desired cell densities for suspension cell immunofluorescence.
These cover-slips can also be used for staining suspension cells for microscopy, or for fixing suspension cells for confocal microscopy. What’s more, you can also stimulate the cells while they are attached on the cover-slip, should you need to do so.
產(chǎn)品訂購信息: